The purpose of this work was to evaluate a potentially useful animal model, Meriones shawi (M.sh)-developing metabolic X syndrome, diabetes and possessing a visual streak similar to human macula-in the study of diabetic retinopathy and diabetic macular edema (DME). Type 2 diabetes (T2D) was induced by high fat diet administration in M.sh. Body weights, blood glucose levels were monitored throughout the study. Diabetic retinal histopathology was evaluated 3 and 7 months after diabetes induction. Retinal thickness was measured, retinal cell types were labeled by immunohistochemistry and the number of stained elements were quantified. Apoptosis was determined with TUNEL assay. T2D induced progressive changes in retinal histology. A significant decrease of retinal thickness and glial reactivity was observed without an increase in apoptosis rate. Photoreceptor outer segment degeneration was evident, with a significant decrease in the number of all cones and M-cone subtype, but-surprisingly-an increase in S-cones. Damage of the pigment epithelium was also confirmed. A decrease in the number and labeling intensity of parvalbumin- and calretinin-positive amacrine cells and a loss of ganglion cells was detected. Other cell types showed no evident alterations. No DME-like condition was noticed even after 7 months. M.sh could be a useful model to study the evolution of diabetic retinal pathology and to identify the role of hypertension and dyslipidemia in the development of the reported alterations. Longer follow up would be needed to evaluate the potential use of the visual streak in modeling human macular diseases.
Pubmed ID: 28542922 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets Calretinin
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets Calbindin D-28k
View all literature mentionsThis monoclonal targets Parvalbumin
View all literature mentionsThis monoclonal targets PRKCA
View all literature mentionsThis monoclonal targets TH
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets S100 beta
View all literature mentionsThis monoclonal targets Vimentin
View all literature mentionsThis polyclonal targets Brn-3a (C-20)
View all literature mentionsThis monoclonal targets Retinal Pigment Epithelium 65
View all literature mentionsThis polyclonal targets Opsin Red/Green
View all literature mentionsThis polyclonal targets Calretinin
View all literature mentions