The incomplete differentiation of human induced pluripotent stem cells (iPSCs) poses a serious safety risk owing to their potential tumorigenicity, hindering their clinical application. Here, we explored the potential of phospho-D-peptides as novel iPSC-eliminating agents. Alkaline phosphatases overexpressed on iPSCs dephosphorylate phospho-D-peptides into hydrophobic peptides that aggregate and induce cell death. We isolated a peptide candidate, D-3, that selectively and rapidly induced toxicity in iPSCs within 1 hr but had little influence on various non-iPSCs, including primary hepatocytes and iPSC-derived cardiomyocytes. Two hours of D-3 treatment efficiently eliminated iPSCs from both single cultures and co-cultures spiked with increasing ratios of iPSCs. In addition, D-3 prevented residual iPSC-induced teratoma formation in a mouse tumorigenicity assay. These results suggest the enormous potential of D-3 as a low-cost and effective anti-iPSC agent for both laboratory use and for the safe clinical application of iPSC-derived cells in regenerative medicine.
Pubmed ID: 28529132 RIS Download
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This monoclonal targets Alkaline Phosphatase
View all literature mentionsThis monoclonal targets TRA-1-60 Antigen
View all literature mentionsThis monoclonal targets Human SSEA-4
View all literature mentionsCell line 692D2 is a Induced pluripotent stem cell with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line 692D2 is a Induced pluripotent stem cell with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Alkaline Phosphatase
View all literature mentionsThis monoclonal targets TRA-1-60 Antigen
View all literature mentionsThis monoclonal targets Human SSEA-4
View all literature mentionsCell line 692D2 is a Induced pluripotent stem cell with a species of origin Homo sapiens (Human)
View all literature mentions