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Microglia, the resident macrophages of the CNS, engage in various CNS-specific functions that are critical for development and health. To better study microglia and the properties that distinguish them from other tissue macrophage populations, we have optimized serum-free culture conditions to permit robust survival of highly ramified adult microglia under defined-medium conditions. We find that astrocyte-derived factors prevent microglial death ex vivo and that this activity results from three primary components, CSF-1/IL-34, TGF-β2, and cholesterol. Using microglial cultures that have never been exposed to serum, we demonstrate a dramatic and lasting change in phagocytic capacity after serum exposure. Finally, we find that mature microglia rapidly lose signature gene expression after isolation, and that this loss can be reversed by engrafting cells back into an intact CNS environment. These data indicate that the specialized gene expression profile of mature microglia requires continuous instructive signaling from the intact CNS.
Pubmed ID: 28521131 RIS Download
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Software package for interpreting gene expression data. Used for interpretation of a large-scale experiment by identifying pathways and processes.
View all literature mentionsAn open community platform for bioimage informatics providing the software resources to visualize, annotate and quantify bioimaging data. To bridge the gap between developers and users, it combines: a) an open-source image analysis software, offering a powerful and flexible environment for developers such as applied mathematicians to write algorithms fast and efficiently; b) a common set of tools to view and manipulate data, and a set of plugins to perform specific quantification or analysis on images; c) a community-based website centralizing all plugins and resources to facilitate their management and maximize their visibility towards users. Workspaces are virtual groups of plugins dedicated to a specific application or image processing domain. By downloading a workspace, ICY automatically installs all corresponding plugins. The workspaces are enabled, but the editing section is not ready yet. If you want to publish a plugin on this website, its code has to be GPL. Source code is available and provided in each application download.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD11b
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis monoclonal targets F4/80 antibody [CI:A3-1]
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets F4/80 antibody [CI:A3-1]
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets F4/80 antibody [CI:A3-1]
View all literature mentionsThis monoclonal targets F4/80 antibody [CI:A3-1]
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets F4/80 antibody [CI:A3-1]
View all literature mentionsThis monoclonal targets F4/80 antibody [CI:A3-1]
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis unknown targets Rabbit IgG
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis monoclonal targets TGF-beta 1,-2,-3
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis monoclonal targets Integrin beta 5
View all literature mentionsThis unknown targets
View all literature mentionsThis unknown targets
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD11b
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD11b
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD11b
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD11b
View all literature mentionsThis monoclonal targets MOUSE ANTI RAT CD11b
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis polyclonal targets Iba1
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