Despite its modest capacity for regeneration, peripheral nervous system injury often results in significant long-term disability. Supplementing peripheral nervous system injury with autologous Schwann cells (SCs) may serve to rejuvenate the postinjury environment to enhance regeneration and ultimately improve functional outcomes. However, human nerve-derived SC (hN-SC) collection procedures require invasive surgical resection. Here, we describe the characterization of SCs from adult human skin (hSk-SCs) of four male donors ranging between 27 and 46 years old. Within five weeks of isolating and culturing adherent mixed skin cells, we were able to obtain 3-5 million purified SCs. We found that hSk-SCs appeared transcriptionally indistinguishable from hN-SCs with both populations exhibiting expression of SC genes including: SOX10, SOX9, AP2A1, CDH19, EGR1, ETV5, PAX3, SOX2, CX32, DHH, NECL4, NFATC4, POU3F1, S100B, and YY1. Phenotypic analysis of hSk-SCs and hN-SCs cultures revealed highly enriched populations of SCs indicated by the high percentage of NES+ve, SOX10+ve, s100+ve and p75+ve cells, as well as the expression of a battery of other SC-associated proteins (PAX3, CDH19, ETV5, SOX2, POU3F1, S100B, EGR2, and YY1). We further show that both hSk-SCs and hN-SCs are capable of promoting axonal growth to similar degrees and that a subset of both associate with regenerating axons and form myelin following transplantation into the injured mouse sciatic nerve. Interestingly, although the majority of both hSk-SCs and hN-SCs maintained SOX10 immunoreactivity following transplant, only a subset of each activated the promyelinating factor, POU3F1, and were able to myelinate. Taken together, we demonstrate that adult hSk-SCs are genetically and phenotypically indistinguishable to hN-SCs.
Pubmed ID: 28512649 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A national mouse monoclonal antibody generating resource for biochemical and immunohistochemical applications in mammalian brain. NeuroMabs are generated from mice immunized with synthetic and recombinant immunogens corresponding to components of the neuronal proteome as predicted from genomic and other large-scale cloning efforts. Comprehensive biochemical and immunohistochemical analyses of human, primate and non-primate mammalian brain are incorporated into the initial NeuroMab screening procedure. This yields a subset of mouse mAbs that are optimized for use in brain (i.e. NeuroMabs): for immunocytochemical-based imaging studies of protein localization in adult, developing and pathological brain samples, for biochemical analyses of subunit composition and post-translational modifications of native brain proteins, and for proteomic analyses of native brain protein networks. The NeuroMab facility was initially funded with a five-year U24 cooperative grant from NINDS and NIMH. The initial goal of the facility for this funding period is to generate a library of novel NeuroMabs against neuronal proteins, initially focusing on membrane proteins (receptors/channels/transporters), synaptic proteins, other neuronal signaling molecules, and proteins with established links to disease states. The scope of the facility was expanded with supplements from the NIH Blueprint for Neuroscience Research to include neurodevelopmental targets, the NIH Roadmap for Medical Research to include epigenetics targets, and NIH Office of Rare Diseases Research to include rare disease targets. These NeuroMabs will then be produced on a large scale and made available to the neuroscience research community on an inexpensive basis as tissue culture supernatants or purified immunoglobulin by Antibodies Inc. The UC Davis/NIH NeuroMab Facility makes NeuroMabs available directly to end users and is unable to accommodate sales to distributors for third party distribution. Note, NeuroMab antibodies are now offered through antibodiesinc.
View all literature mentionsCommercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsMus musculus with name NOD.CB17-Prkdcscid/NcrCrl from IMSR.
View all literature mentionsThis polyclonal targets Krox-20
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsThis polyclonal targets Rat CNTF
View all literature mentionsThis monoclonal targets Caspr
View all literature mentionsThis polyclonal targets Human p75
View all literature mentionsThis polyclonal targets CDH19
View all literature mentionsThis monoclonal targets Fibronectin
View all literature mentionsThis polyclonal targets GFP (Green Fluorescent Protein)
View all literature mentionsThis monoclonal targets Human ETV5 MAb (Clone 654404)
View all literature mentionsThis unknown targets Myelin Basic Protein (36-50) (MBP)
View all literature mentionsThis monoclonal targets Nestin (Rat-401) Mouse mAb
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis polyclonal targets Myelin Protein Zero (Pz0)
View all literature mentionsThis polyclonal targets Pax3
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets S100B
View all literature mentionsThis polyclonal targets S100 isolated from cow brain.
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis monoclonal targets YY1
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsThis polyclonal targets Rat CNTF
View all literature mentionsThis monoclonal targets Caspr
View all literature mentionsThis polyclonal targets Human p75
View all literature mentionsThis polyclonal targets CDH19
View all literature mentionsThis monoclonal targets Fibronectin
View all literature mentionsThis polyclonal targets GFP (Green Fluorescent Protein)
View all literature mentionsThis monoclonal targets Human ETV5 MAb (Clone 654404)
View all literature mentionsThis unknown targets Myelin Basic Protein (36-50) (MBP)
View all literature mentionsThis monoclonal targets Nestin (Rat-401) Mouse mAb
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis polyclonal targets Myelin Protein Zero (Pz0)
View all literature mentionsThis polyclonal targets Pax3
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets S100B
View all literature mentionsThis polyclonal targets S100 isolated from cow brain.
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis monoclonal targets YY1
View all literature mentionsThis polyclonal targets Krox-20
View all literature mentions