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To investigate the role of immune cells in lung regeneration, we used a unilateral pneumonectomy model that promotes the formation of new alveoli in the remaining lobes. Immunofluorescence and single-cell RNA sequencing found CD115+ and CCR2+ monocytes and M2-like macrophages accumulating in the lung during the peak of type 2 alveolar epithelial stem cell (AEC2) proliferation. Genetic loss of function in mice and adoptive transfer studies revealed that bone marrow-derived macrophages (BMDMs) traffic to the lung through a CCL2-CCR2 chemokine axis and are required for optimal lung regeneration, along with Il4ra-expressing leukocytes. Our data suggest that these cells modulate AEC2 proliferation and differentiation. Finally, we provide evidence that group 2 innate lymphoid cells are a source of IL-13, which promotes lung regeneration. Together, our data highlight the potential for immunomodulatory therapies to stimulate alveologenesis in adults.
Pubmed ID: 28506464 RIS Download
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Java toolset for working with next generation sequencing data in the BAM format.
View all literature mentionsScript distributed with the HT-Seq Python framework for processing RNA-seq or DNA-seq data.
View all literature mentionsThis monoclonal targets Mouse T1 / ST2
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets Siglec-F
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD16 / CD32
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis monoclonal targets Gata-3
View all literature mentionsThis monoclonal targets Fc epsilon RI alpha
View all literature mentionsThis monoclonal targets KLRG1
View all literature mentionsThis monoclonal targets CD90.2
View all literature mentionsThis monoclonal targets CD25
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets Ly-6G Ly-6C
View all literature mentionsThis monoclonal targets NK-1.1
View all literature mentionsThis monoclonal targets CD19
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets CD3
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgY (IgG) (H+L)
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgG (H+L)
View all literature mentionsThis monoclonal targets Ly-6G
View all literature mentionsThis monoclonal targets F4/80
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis polyclonal targets RFP
View all literature mentionsThis polyclonal targets RFP
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets Podoplanin
View all literature mentionsThis polyclonal targets Prosurfactant Protein C
View all literature mentionsThis monoclonal targets RAGE
View all literature mentionsThis monoclonal targets Mouse Thymus / Spleen The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes.
View all literature mentionsThis monoclonal targets Ly-6G
View all literature mentionsThis monoclonal targets Mouse F4/80 ANTIGEN
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsSoftware package for quantifying gene and isoform abundances from single end or paired end RNA Seq data. Accurate transcript quantification from RNA Seq data with or without reference genome. Used for accurate quantification of gene and isoform expression from RNA-Seq data.
View all literature mentionsSoftware tool for transcriptome assembly and differential expression analysis for RNA-Seq. Includes script called cuffmerge that can be used to merge together several Cufflinks assemblies. It also handles running Cuffcompare as well as automatically filtering a number of transfrags that are likely to be artifacts. If the researcher has a reference GTF file, the researcher can provide it to the script to more effectively merge novel isoforms and maximize overall assembly quality.
View all literature mentionsSoftware tool for fast and high throughput alignment of shotgun cDNA sequencing reads generated by transcriptomics technologies. Fast splice junction mapper for RNA-Seq reads. Aligns RNA-Seq reads to mammalian-sized genomes using ultra high-throughput short read aligner Bowtie, and then analyzes mapping results to identify splice junctions between exons.TopHat2 is accurate alignment of transcriptomes in presence of insertions, deletions and gene fusions.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
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Mus musculus with name B6.SJL-PtprcaPepc
Mus musculus with name C.129S4(Cg)-Il13tm2.1Lky/J from IMSR.
View all literature mentionsMus musculus with name BALB/c-Il4ratm1Sz/J from IMSR.
View all literature mentionsMus musculus with name B6.129S4-Arg1tm1.1Lky/J from IMSR.
View all literature mentionsMus musculus with name B6.129S-Sftpctm1(cre/ERT2)Blh/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2(Cg)-Cx3cr1tm1Litt/J from IMSR.
View all literature mentionsMus musculus with name B6.129(Cg)-Ccr2tm2.1Ifc/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Tg(Csf1r-EGFP)1Hume/J from IMSR.
View all literature mentionsThis polyclonal targets Prosurfactant Protein C
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