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RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing.
Pubmed ID: 28486133 RIS Download
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Software tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
View all literature mentionsDigital microscopy software for research microscopy. It comes standard with drivers to control numerous instruments in and around the microscope. When online, data is acquired in a native-3D format over time, color and specimen locations in customizable experiment protocols. Data can be analyzed by a wide variety of tools for image processing including mathematical operations, statistics functions, analysis scripting and import to/export from MATLAB. Additional modules are available for special applications ranging from deconvolution to photomanipulation to multiphoton.
View all literature mentionsCell line U2OS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BS-C-1 is a Spontaneously immortalized cell line with a species of origin Chlorocebus pygerythrus (Vervet monkey)
View all literature mentionsCell line U2OS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis unknown targets ROCK2 (D1B1) Rabbit mAb
View all literature mentionsThis polyclonal targets Human PAK5
View all literature mentionsThis monoclonal targets RhoA (67B9) Rabbit mAb
View all literature mentionsThis monoclonal targets Mcm7 antibody produced in mouse
View all literature mentionsThis monoclonal targets ROCK1
View all literature mentionsThis polyclonal targets Phospho-MYPT1 (Thr696)
View all literature mentionsThis monoclonal targets MYPT1 (D6C1) Rabbit mAb
View all literature mentionsThis monoclonal targets Rhoc
View all literature mentionsThis polyclonal targets Zip Kinase antibody produced in rabbit
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis monoclonal targets GRB2
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H + L)
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Ser19)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis monoclonal targets polyHistidine
View all literature mentionsThis polyclonal targets RHOD
View all literature mentionsThis unknown targets Glutathione-S-Transferase (GST)
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H + L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H + L)
View all literature mentionsThis monoclonal targets GRB2
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal targets Human PAK5
View all literature mentionsThis monoclonal targets GRB2
View all literature mentionsThis unknown targets ROCK2 (D1B1) Rabbit mAb
View all literature mentionsThis polyclonal targets Human PAK5
View all literature mentionsThis monoclonal targets ROCK1
View all literature mentionsThis unknown targets ROCK2 (D1B1) Rabbit mAb
View all literature mentionsThis monoclonal targets Rhoc
View all literature mentionsThis monoclonal targets ROCK1
View all literature mentionsThis monoclonal targets RhoA (67B9) Rabbit mAb
View all literature mentionsThis monoclonal targets Rhoc
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Ser19)
View all literature mentionsThis monoclonal targets RhoA (67B9) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-MYPT1 (Thr696)
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Ser19)
View all literature mentionsThis monoclonal targets MYPT1 (D6C1) Rabbit mAb
View all literature mentionsThis polyclonal targets Zip Kinase antibody produced in rabbit
View all literature mentionsThis polyclonal targets Phospho-MYPT1 (Thr696)
View all literature mentionsThis polyclonal targets Zip Kinase antibody produced in rabbit
View all literature mentionsThis monoclonal targets MYPT1 (D6C1) Rabbit mAb
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis monoclonal targets Mcm7 antibody produced in mouse
View all literature mentionsThis monoclonal targets polyHistidine
View all literature mentionsThis monoclonal targets Mcm7 antibody produced in mouse
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis monoclonal targets polyHistidine
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H + L)
View all literature mentionsThis monoclonal targets ROCK1
View all literature mentionsThis monoclonal targets Rhoc
View all literature mentionsThis monoclonal targets RhoA (67B9) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-Myosin Light Chain 2 (Ser19)
View all literature mentionsThis monoclonal targets GRB2
View all literature mentionsThis polyclonal targets Phospho-MYPT1 (Thr696)
View all literature mentionsThis polyclonal targets Human PAK5
View all literature mentionsThis monoclonal targets MYPT1 (D6C1) Rabbit mAb
View all literature mentionsThis polyclonal targets Zip Kinase antibody produced in rabbit
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis unknown targets ROCK2 (D1B1) Rabbit mAb
View all literature mentionsThis monoclonal targets Mcm7 antibody produced in mouse
View all literature mentionsThis monoclonal targets polyHistidine
View all literature mentionsThis unknown targets Glutathione-S-Transferase (GST)
View all literature mentionsThis monoclonal targets β-Actin
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