The concerted production of neurons and glia by neural stem cells (NSCs) is essential for neural circuit assembly. In the developing cerebral cortex, radial glia progenitors (RGPs) generate nearly all neocortical neurons and certain glia lineages. RGP proliferation behavior shows a high degree of non-stochasticity, thus a deterministic characteristic of neuron and glia production. However, the cellular and molecular mechanisms controlling RGP behavior and proliferation dynamics in neurogenesis and glia generation remain unknown. By using mosaic analysis with double markers (MADM)-based genetic paradigms enabling the sparse and global knockout with unprecedented single-cell resolution, we identified Lgl1 as a critical regulatory component. We uncover Lgl1-dependent tissue-wide community effects required for embryonic cortical neurogenesis and novel cell-autonomous Lgl1 functions controlling RGP-mediated glia genesis and postnatal NSC behavior. These results suggest that NSC-mediated neuron and glia production is tightly regulated through the concerted interplay of sequential Lgl1-dependent global and cell intrinsic mechanisms.
Pubmed ID: 28472654 RIS Download
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Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsAllen Mouse Brain Atlas includes full color, high resolution anatomic reference atlas accompanied by systematic, hierarchically organized taxonomy of mouse brain structures. Enables interactive online exploration of atlas and to provide deeper level of 3D annotation for informatics analysis and viewing in Brain Explorer 3D viewer.
View all literature mentionsAllen Mouse Brain Atlas includes full color, high resolution anatomic reference atlas accompanied by systematic, hierarchically organized taxonomy of mouse brain structures. Enables interactive online exploration of atlas and to provide deeper level of 3D annotation for informatics analysis and viewing in Brain Explorer 3D viewer.
View all literature mentionsThis polyclonal targets Rabbit IgG
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgY (IgG) (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis unknown targets Tubulin, gamma
View all literature mentionsThis polyclonal targets EAAT1/SLC1A3 / GLAST1
View all literature mentionsThis polyclonal targets ACTIVE& x00AE; Caspase-3
View all literature mentionsThis monoclonal targets N-cadherin
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View all literature mentionsThis monoclonal targets Ctip2
View all literature mentionsThis monoclonal targets Human SATB2
View all literature mentionsThis monoclonal targets S-100 (beta-Subunit)
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View all literature mentionsThis unknown targets Prokaryotic recombinant fusion protein corresponding to a 1086bp Ki67 motif-containing cDNA fragment
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets RFP / DsRed
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsMus musculus with name 129S1/Sv-Hprt1tm1(CAG-cre)Mnn/J from IMSR.
View all literature mentionsMus musculus with name B6;CBA-Tg(Emx1-cre/ERT2)1Kess/SshiJ from IMSR.
View all literature mentionsMus musculus with name B6.129S2-Emx1tm1(cre)Krj/J from IMSR.
View all literature mentionsMus musculus with name STOCK Igs2tm2(ACTB-tdTomato.-EGFP)Luo/J from IMSR.
View all literature mentionsMus musculus with name STOCK Igs2tm1(ACTB-EGFP.-tdTomato)Luo/J from IMSR.
View all literature mentionsThis polyclonal targets RFP / DsRed
View all literature mentionsMus musculus with name 129S1/Sv-Hprt1tm1(CAG-cre)Mnn/J from IMSR.
View all literature mentionsMus musculus with name B6;CBA-Tg(Emx1-cre/ERT2)1Kess/SshiJ from IMSR.
View all literature mentionsMus musculus with name B6.129S2-Emx1tm1(cre)Krj/J from IMSR.
View all literature mentionsMus musculus with name STOCK Igs2tm2(ACTB-tdTomato.-EGFP)Luo/J from IMSR.
View all literature mentionsMus musculus with name STOCK Igs2tm1(ACTB-EGFP.-tdTomato)Luo/J from IMSR.
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgY (IgG) (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis unknown targets Tubulin, gamma
View all literature mentionsThis polyclonal targets EAAT1/SLC1A3 / GLAST1
View all literature mentionsThis polyclonal targets ACTIVE& x00AE; Caspase-3
View all literature mentionsThis monoclonal targets N-cadherin
View all literature mentionsThis unknown targets Pax-6
View all literature mentionsThis monoclonal targets Ctip2
View all literature mentionsThis monoclonal targets Human SATB2
View all literature mentionsThis monoclonal targets S-100 (beta-Subunit)
View all literature mentionsThis unknown targets
View all literature mentionsThis unknown targets Prokaryotic recombinant fusion protein corresponding to a 1086bp Ki67 motif-containing cDNA fragment
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets Rabbit IgG
View all literature mentionsThis polyclonal targets Rabbit IgG
View all literature mentions