During development, newborn interneurons migrate throughout the embryonic brain. Here, we provide evidence that these interneurons act in a paracrine fashion to regulate developmental oligodendrocyte formation. Specifically, we show that medial ganglionic eminence (MGE) interneurons secrete factors that promote genesis of oligodendrocytes from glially biased cortical precursors in culture. Moreover, when MGE interneurons are genetically ablated in vivo prior to their migration, this causes a deficit in cortical oligodendrogenesis. Modeling of the interneuron-precursor paracrine interaction using transcriptome data identifies the cytokine fractalkine as responsible for the pro-oligodendrocyte effect in culture. This paracrine interaction is important in vivo, since knockdown of the fractalkine receptor CX3CR1 in embryonic cortical precursors, or constitutive knockout of CX3CR1, causes decreased numbers of oligodendrocyte progenitor cells (OPCs) and oligodendrocytes in the postnatal cortex. Thus, in addition to their role in regulating neuronal excitability, interneurons act in a paracrine fashion to promote the developmental genesis of oligodendrocytes.
Pubmed ID: 28472653 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This monoclonal targets Sox2 (C70B1)
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsThis polyclonal targets Mouse Mer Affinity Purified Ab
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis unknown targets Tubulin beta-3
View all literature mentionsThis polyclonal targets Mouse Axl
View all literature mentionsThis polyclonal targets Mouse Dtk
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis unknown targets Rabbit anti mouse fractalkine / neurotactin pAb
View all literature mentionsThis unknown targets Rabbit anti rat CX3CR1 pAb
View all literature mentionsThis polyclonal targets Caspase 3 active (cleaved) form
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets APC
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis polyclonal targets PDGFRA
View all literature mentionsThis monoclonal targets Olig2
View all literature mentionsThis polyclonal targets Oligodendrocute transcription factor 2
View all literature mentionsThis monoclonal targets Nestin clone 10C2
View all literature mentionsThis monoclonal targets Myelin Basic Protein (MBP), aa 82-87
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis unknown targets TurboGFP
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Bovine brain corpus callosum white matter
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsVector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsProgramming language for all operating systems that lets users work more quickly and integrate their systems more effectively. Often compared to Tcl, Perl, Ruby, Scheme or Java. Some of its key distinguishing features include very clear and readable syntax, strong introspection capabilities, intuitive object orientation, natural expression of procedural code, full modularity, exception-based error handling, high level dynamic data types, extensive standard libraries and third party modules for virtually every task, extensions and modules easily written in C, C (or Java for Python, or .NET languages for IronPython), and embeddable within applications as a scripting interface.
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsVector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsProgramming language for all operating systems that lets users work more quickly and integrate their systems more effectively. Often compared to Tcl, Perl, Ruby, Scheme or Java. Some of its key distinguishing features include very clear and readable syntax, strong introspection capabilities, intuitive object orientation, natural expression of procedural code, full modularity, exception-based error handling, high level dynamic data types, extensive standard libraries and third party modules for virtually every task, extensions and modules easily written in C, C (or Java for Python, or .NET languages for IronPython), and embeddable within applications as a scripting interface.
View all literature mentionsMus musculus with name B6.129P2(Cg)-Cx3cr1tm1Litt/J from IMSR.
View all literature mentionsMus musculus with name B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J from IMSR.
View all literature mentionsMus musculus with name B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J-Tg(Nkx2-1-cre)2Sand/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J from IMSR.
View all literature mentionsThis polyclonal targets Mouse Mer Affinity Purified Ab
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis unknown targets Tubulin beta-3
View all literature mentionsMus musculus with name B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J from IMSR.
View all literature mentionsMus musculus with name B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J-Tg(Nkx2-1-cre)2Sand/J from IMSR.
View all literature mentionsThis polyclonal targets GFAP
View all literature mentionsMus musculus with name B6.129P2(Cg)-Cx3cr1tm1Litt/J from IMSR.
View all literature mentionsThis monoclonal targets Myelin Basic Protein (MBP), aa 82-87
View all literature mentionsThis monoclonal targets Nestin clone 10C2
View all literature mentionsThis polyclonal targets PDGFRA
View all literature mentionsThis monoclonal targets Olig2
View all literature mentionsThis polyclonal targets Oligodendrocute transcription factor 2
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis monoclonal targets APC
View all literature mentionsMus musculus with name B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J from IMSR.
View all literature mentionsThis polyclonal targets Mouse Dtk
View all literature mentionsThis polyclonal targets Mouse Axl
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis unknown targets Rabbit anti mouse fractalkine / neurotactin pAb
View all literature mentionsThis unknown targets Rabbit anti rat CX3CR1 pAb
View all literature mentionsThis polyclonal targets Caspase 3 active (cleaved) form
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis unknown targets TurboGFP
View all literature mentionsThis polyclonal targets GFP
View all literature mentions