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The proneural transcription factor Neurogenin3 (Ngn3) plays a critical role in pancreatic endocrine cell differentiation, although regulation of Ngn3 protein is largely unexplored. Here we demonstrate that Ngn3 protein undergoes cyclin-dependent kinase (Cdk)-mediated phosphorylation on multiple serine-proline sites. Replacing wild-type protein with a phosphomutant form of Ngn3 increases α cell generation, the earliest endocrine cell type to be formed in the developing pancreas. Moreover, un(der)phosphorylated Ngn3 maintains insulin expression in adult β cells in the presence of elevated c-Myc and enhances endocrine specification during ductal reprogramming. Mechanistically, preventing multi-site phosphorylation enhances both Ngn3 stability and DNA binding, promoting the increased expression of target genes that drive differentiation. Therefore, multi-site phosphorylation of Ngn3 controls its ability to promote pancreatic endocrine differentiation and to maintain β cell function in the presence of pro-proliferation cues and could be manipulated to promote and maintain endocrine differentiation in vitro and in vivo.
Pubmed ID: 28457793 RIS Download
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Software repository for R packages related to analysis and comprehension of high throughput genomic data. Uses separate set of commands for installation of packages. Software project based on R programming language that provides tools for analysis and comprehension of high throughput genomic data.
View all literature mentionsCommercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsSoftware package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 28,2023. Software Python package that provides infrastructure to process data from high-throughput sequencing assays. While the main purpose of HTSeq is to allow you to write your own analysis scripts, customized to your needs, there are also a couple of stand-alone scripts for common tasks that can be used without any Python knowledge.
View all literature mentionsCompany offering a broad range of instrument systems and diagnostic tests for hospitals, reference labs, blood banks, physician offices and clinics to aid in the diagnosis of a range of serious health issues such as infectious diseases, cancer, and diabetes, as well as monitor other important indicators of health.
View all literature mentionsSoftware package for low-level analyses of single-cell RNA-seq data. Used for quality control, data exploration and normalization, cell cycle phase assignment, identification of highly variable and correlated genes, clustering into subpopulations and marker gene detection.
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets CD326 (EpCAM)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets HA tag - ChIP Grade
View all literature mentionsThis monoclonal targets neurogenin 3
View all literature mentionsThis polyclonal targets Neurogenin 3
View all literature mentionsThis polyclonal targets Human Pancreatic Polypeptide
View all literature mentionsThis polyclonal targets Glucagon
View all literature mentionsThis monoclonal targets Glucagon antibody [K79bB10]
View all literature mentionsThis polyclonal targets Insulin
View all literature mentionsAllele Detail: Transgenic This is a legacy resource.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsCell line MIN6 is a Transformed cell line with a species of origin Mus musculus
View all literature mentionsCell line mPAC L20 is a Transformed cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsAllele Detail: Transgenic This is a legacy resource.
View all literature mentionsThis polyclonal targets HA tag - ChIP Grade
View all literature mentionsThis monoclonal targets neurogenin 3
View all literature mentionsThis polyclonal targets Human Pancreatic Polypeptide
View all literature mentionsAllele Detail: Transgenic This is a legacy resource.
View all literature mentionsThis monoclonal targets CD326 (EpCAM)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets Neurogenin 3
View all literature mentionsThis polyclonal targets Glucagon
View all literature mentionsThis monoclonal targets Glucagon antibody [K79bB10]
View all literature mentionsThis polyclonal targets Insulin
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