Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Perturbation of Serotonin Homeostasis during Adulthood Affects Serotonergic Neuronal Circuitry.

eNeuro |

Growing evidence shows that the neurotransmitter serotonin (5-HT) modulates the fine-tuning of neuron development and the establishment of wiring patterns in the brain. However, whether serotonin is involved in the maintenance of neuronal circuitry in the adult brain remains elusive. Here, we use aconditional knockout (cKO) mouse line to assess the impact of serotonin depletion during adulthood on serotonergic system organization. Data show that the density of serotonergic fibers is increased in the hippocampus and decreased in the thalamic paraventricular nucleus (PVN) as a consequence of brain serotonin depletion. Strikingly, these defects are rescued following reestablishment of brain 5-HT signaling via administration of the serotonin precursor 5-hydroxytryptophan (5-HTP). Finally, 3D reconstruction of serotonergic fibers reveals that changes in serotonin homeostasis affect axonal branching complexity. These data demonstrate that maintaining proper serotonin homeostasis in the adult brain is crucial to preserve the correct serotonergic axonal wiring.

Pubmed ID: 28413824 RIS Download

Mesh terms: 5-Hydroxytryptophan | Animals | Brain-Derived Neurotrophic Factor | Gene Expression Regulation | Green Fluorescent Proteins | Homeostasis | Mice | Mice, Inbred C57BL | Mice, Transgenic | Midline Thalamic Nuclei | Nerve Net | Neural Pathways | RNA, Messenger | Serotonergic Neurons | Serotonin | Serotonin Agents | Serotonin Plasma Membrane Transport Proteins | Signal Transduction | Tryptophan Hydroxylase

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Thermo Fisher Scientific (tool)

RRID:SCR_008452

Commercial vendor and service provider of laboratory reagents and antibodies.

View all literature mentions

Trk B (H-181) antibody (antibody)

RRID:AB_2155274

This polyclonal antibody targets Trk B (H-181)

View all literature mentions

Anti-Serotonin Transporter antibody (antibody)

RRID:AB_612176

This polyclonal antibody targets Serotonin Transporter

View all literature mentions

Anti-GFP antibody (antibody)

RRID:AB_300798

This polyclonal antibody targets GFP

View all literature mentions

Rabbit Anti-Green Fluorescent Protein (GFP) Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_221570

This polyclonal antibody targets Green Fluorescent Protein (GFP)

View all literature mentions

Anti-α-Tubulin antibody (antibody)

RRID:AB_477593

This monoclonal antibody targets α-Tubulin

View all literature mentions

BDNF (N-20) antibody (antibody)

RRID:AB_630940

This monoclonal antibody targets BDNF

View all literature mentions

Anti-Serotonin antibody produced in rabbit (antibody)

RRID:AB_477522

This polyclonal antibody targets Serotonin antibody produced in rabbit

View all literature mentions

GAPDH (6C5) antibody (antibody)

RRID:AB_627679

This monoclonal antibody targets GAPDH (6C5)

View all literature mentions

FilamentTracer (tool)

RRID:SCR_007366

A software application that automatically detects neurons (dendritic trees, axons and spines), microtubules, and other filament-like structures in 2D, 3D and 4D. Imaris FilamentTracer uses strategies that involve an optimal combination of automated analysis and operator decision. The choice of the best method is an essential element in optimizing efficiency. FilamentTracer allows the choice between four complementary tracing strategies ranging from manual to automatic and utilizes a creation wizard to easily guide the user through the creations steps. It has been specifically designed to deal with the complex problem of spine analysis over time.

View all literature mentions

ImageJ (tool)

RRID:SCR_003070

Software tool as an open source Java-based image processing program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions

Imaris (tool)

RRID:SCR_007370

Interactive Vision In The Life SciencesCombining speed, precision and ease-of-use, Imaris provides an impressive range of capabilities for working with three-dimensional images. Move easily between simple and advanced 3D views. Use flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.BitPlane, Inc. also releases the following companion products:- ImarisXT is a multi-functional two-way interface from Imaris to both classic programming languages and to Matlab.- Imaris MeasurementPro and Imaris Track provide the ability to segment and follow the movement of independent objects in a 3D image over time. Objects can either be Surface Objects or Spots, created automatically or manually.- ImarisColoc enables users to easily isolate, visualize, and quantify regional overlap in 3D and 4D images and automates the process of selecting colocalized image areas.

View all literature mentions

Quantity One 1-D Analysis Software (tool)

RRID:SCR_014280

Software used for Bio-Rad imaging systems to acquire, quantitate, and analyze a variety of data. The software allows automatic configuration of imaging systems with appropriate filters, lasers, LEDs, and other illumination sources. It also contains tools for automated analysis of tests and assays such as 1-D electrophoretic gels, western blots, and colony counts.

View all literature mentions