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Neuronal activity is regulated in a homeostatic manner through changes in inhibitory GABA and excitatory glutamate (Glu) AMPA (A) receptors (GluARs). Using immunofluorescent staining, we examined whether calcium/calmodulin-dependent protein kinase IIα (CaMKIIα)-labeled (+) excitatory neurons in the barrel cortex undergo such homeostatic regulation following enforced waking with associated cortical activation during the day when mice normally sleep the majority of the time. Sleep deprived mice were prevented from falling asleep by unilateral whisker stimulation and sleep recovery (SR) mice allowed to sleep freely following deprivation. In parallel with changes in c-Fos reflecting changes in activity, (β2-3 subunits of) GABAA Rs were increased on the membrane of CaMKIIα+ neurons with enforced waking and returned to baseline levels with SR in barrel cortex on sides both contra- and ipsilateral to the whisker stimulation. The GABAAR increase was correlated with increased gamma electroencephalographic (EEG) activity across conditions. On the other hand, (GluA1 subunits of) AMPA Rs were progressively removed from the membrane of CaMKIIα+ neurons by (Rab5+) early endosomes during enforced waking and returned to the membrane by (Rab11+) recycling endosomes during SR. The internalization of the GluA1Rs paralleled the expression of Arc, which mediates homeostatic regulation of AMPA receptors through an endocytic pathway. The reciprocal changes in GluA1Rs relative to GABAARs suggest homeostatic down-scaling during enforced waking and sensory stimulation and restorative up-scaling during recovery sleep. Such homeostatic changes with sleep-wake states and their associated cortical activities could stabilize excitability and activity in excitatory cortical neurons.
Pubmed ID: 28408870 RIS Download
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View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Rab11 antibody
View all literature mentionsThis polyclonal targets Rab5 antibody - Early Endosome Marker
View all literature mentionsThis monoclonal targets Rab11 antibody [KT80]
View all literature mentionsThis monoclonal targets Rab5 antibody [3A4]
View all literature mentionsThis polyclonal targets Glutamate receptor 1
View all literature mentionsThis monoclonal targets GABA A Receptor beta 23 Chain extracellular domain of the beta2 & beta3 subunits of the GABA A receptor clone BD17
View all literature mentionsThis polyclonal targets c-Fos
View all literature mentionsThis unknown targets CaMKII alpha
View all literature mentionsThis monoclonal targets CaM Kinase IIalpha (CaMKIIalpha)
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Rab11 antibody
View all literature mentionsThis polyclonal targets Rab5 antibody - Early Endosome Marker
View all literature mentionsThis monoclonal targets Rab11 antibody [KT80]
View all literature mentionsThis monoclonal targets Rab5 antibody [3A4]
View all literature mentionsThis polyclonal targets Glutamate receptor 1
View all literature mentionsThis monoclonal targets GABA A Receptor beta 23 Chain extracellular domain of the beta2 & beta3 subunits of the GABA A receptor clone BD17
View all literature mentionsThis polyclonal targets c-Fos
View all literature mentionsThis unknown targets CaMKII alpha
View all literature mentionsThis monoclonal targets CaM Kinase IIalpha (CaMKIIalpha)
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Rab11 antibody
View all literature mentionsThis polyclonal targets Rab5 antibody - Early Endosome Marker
View all literature mentionsThis monoclonal targets Rab11 antibody [KT80]
View all literature mentionsThis monoclonal targets Rab5 antibody [3A4]
View all literature mentionsThis polyclonal targets Glutamate receptor 1
View all literature mentionsThis monoclonal targets GABA A Receptor beta 23 Chain extracellular domain of the beta2 & beta3 subunits of the GABA A receptor clone BD17
View all literature mentionsThis polyclonal targets c-Fos
View all literature mentionsThis unknown targets CaMKII alpha
View all literature mentionsThis monoclonal targets CaM Kinase IIalpha (CaMKIIalpha)
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