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An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells.

Cell | Apr 6, 2017

Cells operate through protein interaction networks organized in space and time. Here, we describe an approach to resolve both dimensions simultaneously by using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to capture entire organelle proteomes with high temporal resolution, but its breadth of labeling is generally thought to preclude the higher spatial resolution necessary to interrogate specific protein networks. We provide a solution to this problem by combining quantitative proteomics with a system of spatial references. As proof of principle, we apply this approach to interrogate proteins engaged by G-protein-coupled receptors as they dynamically signal and traffic in response to ligand-induced activation. The method resolves known binding partners, as well as previously unidentified network components. Validating its utility as a discovery pipeline, we establish that two of these proteins promote ubiquitin-linked receptor downregulation after prolonged activation.

Pubmed ID: 28388416 RIS Download

Mesh terms: Animals | Ascorbate Peroxidases | Humans | Lysosomes | Protein Interaction Maps | Protein Transport | Receptors, G-Protein-Coupled | Receptors, Opioid | Staining and Labeling | Ubiquitin

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Associated grants

  • Agency: NHLBI NIH HHS, Id: P01 HL089707
  • Agency: NIGMS NIH HHS, Id: T32 GM007810
  • Agency: NIDA NIH HHS, Id: R37 DA010711
  • Agency: NIAID NIH HHS, Id: R01 AI120694
  • Agency: NIDA NIH HHS, Id: F32 DA038947
  • Agency: NIDA NIH HHS, Id: R29 DA010711
  • Agency: NCI NIH HHS, Id: R01 CA186568
  • Agency: NIGMS NIH HHS, Id: P50 GM082250
  • Agency: NIAID NIH HHS, Id: U19 AI118610
  • Agency: NIAID NIH HHS, Id: P30 AI027763
  • Agency: NIAID NIH HHS, Id: U19 AI106754
  • Agency: NIAID NIH HHS, Id: P01 AI063302
  • Agency: NIDA NIH HHS, Id: R01 DA012864
  • Agency: NIDA NIH HHS, Id: R01 DA010711

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