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Mitochondrial Patch Clamp of Beige Adipocytes Reveals UCP1-Positive and UCP1-Negative Cells Both Exhibiting Futile Creatine Cycling.

Cell metabolism | Apr 4, 2017

Cold and other environmental factors induce "browning" of white fat depots-development of beige adipocytes with morphological and functional resemblance to brown fat. Similar to brown fat, beige adipocytes are assumed to express mitochondrial uncoupling protein 1 (UCP1) and are thermogenic due to the UCP1-mediated H+ leak across the inner mitochondrial membrane. However, this assumption has never been tested directly. Herein we patch clamped the inner mitochondrial membrane of beige and brown fat to provide a direct comparison of their thermogenic H+ leak (IH). All inguinal beige adipocytes had robust UCP1-dependent IH comparable to brown fat, but it was about three times less sensitive to purine nucleotide inhibition. Strikingly, only ∼15% of epididymal beige adipocytes had IH, while in the rest UCP1-dependent IH was undetectable. Despite the absence of UCP1 in the majority of epididymal beige adipocytes, these cells employ prominent creatine cycling as a UCP1-independent thermogenic mechanism.

Pubmed ID: 28380374 RIS Download

Mesh terms: Adipocytes, Beige | Adipose Tissue, Brown | Animals | Cell Respiration | Creatine | Epididymis | Fatty Acids | Inguinal Canal | Male | Mice | Mitochondria | Oxidative Phosphorylation | Patch-Clamp Techniques | Protons | Purine Nucleotides | Receptors, Adrenergic, beta-3 | Uncoupling Protein 1

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