Organ fitness depends on appropriate maintenance of stem cell populations, and aberrations in functional stem cell numbers are associated with malignancies and aging. Symmetrical division is the best characterized mechanism of stem cell replacement, but other mechanisms could also be deployed, particularly in situations of high stress. Here, we show that after severe depletion, intestinal stem cells (ISCs) in the Drosophila midgut are replaced by spindle-independent ploidy reduction of cells in the enterocyte lineage through a process known as amitosis. Amitosis is also induced by the functional loss of ISCs coupled with tissue demand and in aging flies, underscoring the generality of this mechanism. However, we also found that random homologous chromosome segregation during ploidy reduction can expose deleterious mutations through loss of heterozygosity. Together, our results highlight amitosis as an unappreciated mechanism for restoring stem cell homeostasis, but one with some associated risk in animals carrying mutations.
Pubmed ID: 28343984 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsThis unknown targets IgG1
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG1
View all literature mentionsThis polyclonal secondary targets IgG2a
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis polyclonal targets Chicken Chicken IgY secondary - H&L
View all literature mentionsThis polyclonal targets Citrine, EGFP, GFP, YFP
View all literature mentionsThis unknown targets phospho-Histone H3 (Ser10)
View all literature mentionsThis monoclonal targets Delta, extracellular domain
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets RAT ANTI TUBULIN ALPHA
View all literature mentionsThis monoclonal targets lamin Dm0
View all literature mentionsThis polyclonal targets RFP / DsRed
View all literature mentionsThis monoclonal targets Prospero protein
View all literature mentionsThis monoclonal targets Rat CD2
View all literature mentionsThis unknown targets ANTIBODY AFFINITY GEL
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=His2Av-mRFP1}III.1 P{w[+mW.hs]=FRT(w[hs])}2A from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR28E03-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name Oregon-R-P2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name kuz[e29-4]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{y[+mDint2] w[+mC]=EPgy2}Akt[EY10012]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-CD2}5 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; betaTub60D[Pin-Yt]/CyO; P{w[+mC]=UAS-mCD8::GFP.L}LL6 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+m*]=NRE-EGFP.S}1 from BDSC.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsSoftware used for visualizing and graphing data, image processing, and programming. It is designed for use by scientists and engineers and supports large data sets, evenly spaced data, and various data import formats. The software includes a suite of image processing operations for image filtering, manipulation, and quantification and is completely programmable.
View all literature mentionsSoftware tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsDrosophila melanogaster with name Oregon-R-P2 from BDSC.
View all literature mentionsDrosophila melanogaster with name kuz[e29-4]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR28E03-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=His2Av-mRFP1}III.1 P{w[+mW.hs]=FRT(w[hs])}2A from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR28E03-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=His2Av-mRFP1}III.1 P{w[+mW.hs]=FRT(w[hs])}2A from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=His2Av-mRFP1}III.1 P{w[+mW.hs]=FRT(w[hs])}2A from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name Oregon-R-P2 from BDSC.
View all literature mentionsDrosophila melanogaster with name kuz[e29-4]/CyO from BDSC.
View all literature mentionsThis unknown targets IgG1
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-CD2}5 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{y[+mDint2] w[+mC]=EPgy2}Akt[EY10012]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; betaTub60D[Pin-Yt]/CyO; P{w[+mC]=UAS-mCD8::GFP.L}LL6 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+m*]=NRE-EGFP.S}1 from BDSC.
View all literature mentionsDrosophila melanogaster with name Oregon-R-P2 from BDSC.
View all literature mentionsDrosophila melanogaster with name kuz[e29-4]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TM6B, P{w[+mC]=tubP-GAL80}OV3, Tb[1]/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR28E03-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=His2Av-mRFP1}III.1 P{w[+mW.hs]=FRT(w[hs])}2A from BDSC.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG1
View all literature mentionsThis unknown targets IgG1
View all literature mentionsThis polyclonal secondary targets IgG2a
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis polyclonal targets Chicken Chicken IgY secondary - H&L
View all literature mentionsThis polyclonal targets Citrine, EGFP, GFP, YFP
View all literature mentionsThis unknown targets phospho-Histone H3 (Ser10)
View all literature mentionsThis monoclonal targets Delta, extracellular domain
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets RAT ANTI TUBULIN ALPHA
View all literature mentionsThis monoclonal targets lamin Dm0
View all literature mentionsThis polyclonal targets RFP / DsRed
View all literature mentionsThis monoclonal targets Prospero protein
View all literature mentionsThis monoclonal targets Rat CD2
View all literature mentionsThis unknown targets ANTIBODY AFFINITY GEL
View all literature mentionsThis polyclonal targets GFP
View all literature mentions