Searching across hundreds of databases
The ubiquitin ligase TRAF6 is a key regulator of canonical IκB kinase (IKK)/NF-κB signaling in response to interleukin-1 (IL-1) stimulation. Here, we identified the deubiquitinating enzyme YOD1 (OTUD2) as a novel interactor of TRAF6 in human cells. YOD1 binds to the C-terminal TRAF homology domain of TRAF6 that also serves as the interaction surface for the adaptor p62/Sequestosome-1, which is required for IL-1 signaling to NF-κB. We show that YOD1 competes with p62 for TRAF6 association and abolishes the sequestration of TRAF6 to cytosolic p62 aggregates by a non-catalytic mechanism. YOD1 associates with TRAF6 in unstimulated cells but is released upon IL-1β stimulation, thereby facilitating TRAF6 auto-ubiquitination as well as NEMO/IKKγ substrate ubiquitination. Further, IL-1 triggered IKK/NF-κB signaling and induction of target genes is decreased by YOD1 overexpression and augmented after YOD1 depletion. Hence, our data define that YOD1 antagonizes TRAF6/p62-dependent IL-1 signaling to NF-κB.
Pubmed ID: 28244869 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A commercial antibody supplier and provider of various services.
View all literature mentionsGlobal nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsNon-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsCompany provides laboratories worldwide with analytical instruments and supplies, clinical and diagnostic testing services, consumables, applications and expertise in life sciences and applied chemical markets.
View all literature mentionsCell line HUVEC-C is a Finite cell line with a species of origin Homo sapiens
View all literature mentionsThis monoclonal targets IkappaB-alpha
View all literature mentionsThis polyclonal targets IKBKG
View all literature mentionsThis polyclonal targets Human IKKg / NEMO / FIP3 (NT)
View all literature mentionsThis polyclonal targets ACTG1, ACTC1, ACTA1, ACTA2, ACTG2, ACTB
View all literature mentionsThis monoclonal targets IkappaB-alpha, phospho (Ser32 / Ser36)
View all literature mentionsThis unknown targets biotin HRP-linked
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets Linear Polyubiquitin
View all literature mentionsThis polyclonal targets SHARPIN
View all literature mentionsThis polyclonal targets Human RNF31 / HOIP
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis unknown targets USP5/IsoT
View all literature mentionsThis polyclonal targets OTULIN / FAM105B
View all literature mentionsThis monoclonal targets CYLD
View all literature mentionsThis polyclonal targets YOD1
View all literature mentionsThis monoclonal targets Glutathione-S-Transferase (GST) antibody produced in mouse
View all literature mentionsThis polyclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis monoclonal targets p62 Lck Ligand
View all literature mentionsThis monoclonal targets Strep-Tag?? II
View all literature mentionsThis monoclonal targets Ubiquitin, Lys63-Specific
View all literature mentionsThis polyclonal targets YOD1 antibody produced in rabbit
View all literature mentionsThis unknown targets Rabbit IgG Control
View all literature mentionsThis monoclonal targets TRAF6
View all literature mentionsThis monoclonal targets SQSTM1 / p62
View all literature mentionsThis monoclonal targets synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK
View all literature mentionsThis polyclonal targets SAPK/JNK
View all literature mentionsThis unknown targets IKK alpha, phospho (Ser180) / IKK beta, phospho (Ser181)
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis polyclonal targets MAPK14
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis polyclonal targets IKBKB, CHUK
View all literature mentionsThis monoclonal targets GAL4-TA (C-10)
View all literature mentionsThis polyclonal targets RELA
View all literature mentionsThis polyclonal targets Mapk1
View all literature mentionsThis monoclonal targets IKK alpha
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsA quantitative proteomics software package for analyzing large-scale mass-spectrometric data sets. It is a set of algorithms that include peak detection and scoring of peptides, mass calibration, database searches for protein identification, protein quantification, and provides summary statistics.
View all literature mentionsA user-sponsored molecular visualization software system on an open-source foundation. The software has the capabilities to view, render, animate, export, present and develop three dimensional molecular structures.
View all literature mentionsSoftware for macromolecular model building, model completion and validation, and protein modelling using X-ray data. Coot displays maps and models and allows model manipulations such as idealization, rigid-body fitting, ligand search, Ramachandran plots, non-crystallographic symmetry and more. Source code is available.
View all literature mentionsCrystallographic software which solves structures using algorithms and automated rapid search calculations to perform molecular replacement and experimental phasing methods.
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets IkappaB-alpha
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsCell line NCTC clone 929 is a Spontaneously immortalized cell line with a species of origin Mus musculus
View all literature mentionsCell line 293-CD40 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line U2OS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line PC-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets IkappaB-alpha
View all literature mentionsThis monoclonal targets IkappaB-alpha
View all literature mentionsCell line NCTC clone 929 is a Spontaneously immortalized cell line with a species of origin Mus musculus
View all literature mentionsCell line 293-CD40 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line U2OS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line PC-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsThis unknown targets IKK alpha, phospho (Ser180) / IKK beta, phospho (Ser181)
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis polyclonal targets IKBKG
View all literature mentionsThis monoclonal targets GAL4-TA (C-10)
View all literature mentionsThis monoclonal targets Strep-Tag?? II
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis monoclonal targets Ubiquitin, Lys63-Specific
View all literature mentionsThis polyclonal targets YOD1
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets SQSTM1 / p62
View all literature mentionsThis polyclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis polyclonal targets Mapk1
View all literature mentionsThis monoclonal targets p62 Lck Ligand
View all literature mentionsThis monoclonal targets IKK alpha
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
