Searching across hundreds of databases
A high density of Na+ channels at nodes of Ranvier is necessary for rapid and efficient action potential propagation in myelinated axons. Na+ channel clustering is thought to depend on two axonal cell adhesion molecules that mediate interactions between the axon and myelinating glia at the nodal gap (i.e., NF186) and the paranodal junction (i.e., Caspr). Here we show that while Na+ channels cluster at nodes in the absence of NF186, they fail to do so in double conditional knockout mice lacking both NF186 and the paranodal cell adhesion molecule Caspr, demonstrating that a paranodal junction-dependent mechanism can cluster Na+ channels at nodes. Furthermore, we show that paranode-dependent clustering of nodal Na+ channels requires axonal βII spectrin which is concentrated at paranodes. Our results reveal that the paranodal junction-dependent mechanism of Na+channel clustering is mediated by the spectrin-based paranodal axonal cytoskeleton.
Pubmed ID: 28134616 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Commercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.
View all literature mentionsCenter that produces knockout mice and carries out high-throughput phenotyping of each line in order to determine function of every gene in mouse genome. These mice will be preserved in repositories and made available to scientific community representing valuable resource for basic scientific research as well as generating new models for human diseases.
View all literature mentionsSoftware tool for basic image acquisition and analysis. Zeiss ZEN microscopy software to control of axiocam microscope cameras, image transformation and measurements, image processing, image alignment for correlative microscopy, various visualization options.
View all literature mentionsThis monoclonal targets Myelin Basic Protein (MBP), aa 82-87
View all literature mentionsThis unknown targets Human/Mouse/Rat Neurofascin Affinity Purified Ab
View all literature mentionsThis polyclonal targets SD domain of beta IV spectrin
View all literature mentionsThis unknown targets NaV1.6 (SCN8A) Channel
View all literature mentionsThis monoclonal targets beta-Tubulin I antibody produced in mouse
View all literature mentionsThis monoclonal targets Kv1.1 K+ channel (external)
View all literature mentionsThis monoclonal targets Ankyrin-G (Staining)
View all literature mentionsThis monoclonal targets Ankyrin-G (Staining)
View all literature mentionsThis monoclonal targets Sodium Channel, Pan
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
