Although infiltrating macrophages influence many pathological processes after spinal cord injury (SCI), the intrinsic molecular mechanisms that regulate their function are poorly understood. A major hurdle has been dissecting macrophage-specific functions from those in other cell types as well as understanding how their functions change over time. Therefore, we used the RiboTag method to obtain macrophage-specific mRNA directly from the injured spinal cord in mice and performed RNA sequencing to investigate their transcriptional profile. Our data show that at 7 d after SCI, macrophages are best described as foam cells, with lipid catabolism representing the main biological process, and canonical nuclear receptor pathways as their potential mediators. Genetic deletion of a lipoprotein receptor, CD36, reduces macrophage lipid content and improves lesion size and locomotor recovery. Therefore, we report the first macrophage-specific transcriptional profile after SCI and highlight the lipid catabolic pathway as an important macrophage function that can be therapeutically targeted after SCI.SIGNIFICANCE STATEMENT The intrinsic molecular mechanisms that regulate macrophage function after spinal cord injury (SCI) are poorly understood. We obtained macrophage-specific mRNA directly from the injured spinal cord and performed RNA sequencing to investigate their transcriptional profile. Our data show that at 7 d after SCI, macrophages are best described as foam cells, with lipid catabolism representing the main biological process and canonical nuclear receptor pathways as their potential mediators. Genetic deletion of a lipoprotein receptor, CD36, reduces macrophage lipid content and improves lesion size and locomotor recovery. Therefore, we report the first macrophage-specific transcriptional profile after SCI and highlight the lipid catabolic pathway as an important macrophage function that can be therapeutically targeted after SCI.
Pubmed ID: 28130359 RIS Download
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This unknown targets isotype control
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis isotype control targets Trinitrophenol + KLH
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View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis monoclonal targets CD86
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD206
View all literature mentionsThis monoclonal targets CD43
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsThis monoclonal targets Ly-6C
View all literature mentionsThis monoclonal targets Ly-6G
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD16/32
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets Mouse CD36/SR-B3 MAb (Clone 324205)
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis unknown targets Rat IgG2b kappa Isotype Control Allophycocyanin Clone RTK4530
View all literature mentionsMus musculus with name B6.129S1-Cd36tm1Mfe/J from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsMus musculus with name B6N.129-Rpl22tm1.1Psam/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2-Lyz2tm1(cre)Ifo/J from IMSR.
View all literature mentionsSoftware package for interpreting gene expression data. Used for interpretation of a large-scale experiment by identifying pathways and processes.
View all literature mentionsSoftware that manages the RNA-sequencing pipeline based on the TopHat suite of software automatically.
View all literature mentionsThis monoclonal targets CD206
View all literature mentionsThis monoclonal targets CD43
View all literature mentionsThis monoclonal targets CD86
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsThis monoclonal targets Ly-6C
View all literature mentionsThis monoclonal targets Ly-6G
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD16/32
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets Mouse CD36/SR-B3 MAb (Clone 324205)
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsMus musculus with name B6.129S1-Cd36tm1Mfe/J from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsMus musculus with name B6N.129-Rpl22tm1.1Psam/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2-Lyz2tm1(cre)Ifo/J from IMSR.
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis unknown targets isotype control
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis isotype control targets Trinitrophenol + KLH
View all literature mentions