Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans. To date, however, whether human PSCs (hPSCs) can contribute to chimera formation in non-rodent species remains unknown. We systematically evaluate the chimeric competency of several types of hPSCs using a more diversified clade of mammals, the ungulates. We find that naïve hPSCs robustly engraft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implantation pig embryos. Instead, an intermediate hPSC type exhibits higher degree of chimerism and is able to generate differentiated progenies in post-implantation pig embryos.
Pubmed ID: 28129541 RIS Download
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Core facility established to assist the Salk community with integrating genomics data into their research. The primary focus of the core is to provide analysis support for next-generation sequencing applications.
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View all literature mentionsCell line CCD-1112Sk is a Finite cell line with a species of origin Homo sapiens (Human)
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Mus musculus with name NOD.Cg-Prkdcscid Il2rg
Mus musculus with name C57BL/6J from IMSR.
View all literature mentionsAn online resource which helps researchers manage and organize labs and experimental results by supplying molecular biology software tools for experimental design and data analysis. Benchling provides tools for functions such as primer design and colony counting as well as CRISPR guide design and automated Gibson and Golden Gate cloning. Users can take notes in line with data, link data across entries, keep files and data in one place, and manage and keep track of team progress. An enterprise version of Benchling is available for scientists working within an organization with additional administrative, compliance, and security protocols.
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis monoclonal targets Oct-3/4
View all literature mentionsThis monoclonal targets 129/Sv mouse-derived endothelioma cell line tEnd.1
View all literature mentionsThis polyclonal targets H3K9me3
View all literature mentionsThis polyclonal targets Histone H4 (tri methyl K20) - ChIP Grade
View all literature mentionsThis monoclonal targets Actin, alpha, Smooth Muscle
View all literature mentionsThis monoclonal targets Human EPCAM
View all literature mentionsThis monoclonal targets MCF7 cell line
View all literature mentionsThis monoclonal targets Keratin, type II; cytokeratin 8/18; EndoA
View all literature mentionsThis monoclonal targets Tubulin beta-3
View all literature mentionsThis monoclonal targets Oct-3/4
View all literature mentionsThis monoclonal targets Actin, alpha, Smooth Muscle
View all literature mentionsThis monoclonal targets Human EPCAM
View all literature mentionsThis monoclonal targets 129/Sv mouse-derived endothelioma cell line tEnd.1
View all literature mentionsThis monoclonal targets MCF7 cell line
View all literature mentionsThis monoclonal targets Keratin, type II; cytokeratin 8/18; EndoA
View all literature mentionsThis polyclonal targets Histone H4 (tri methyl K20) - ChIP Grade
View all literature mentionsThis polyclonal targets H3K9me3
View all literature mentions