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Signaling networks downstream of receptor tyrosine kinases are among the most extensively studied biological networks, but new approaches are needed to elucidate causal relationships between network components and understand how such relationships are influenced by biological context and disease. Here, we investigate the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches. We focus on a well-defined set of signaling proteins profiled under inhibition with five kinase inhibitors in 32 contexts: four breast cancer cell lines (MCF7, UACC812, BT20, and BT549) under eight stimulus conditions. The data, spanning multiple pathways and comprising ∼70,000 phosphoprotein and ∼260,000 protein measurements, provide a wealth of testable, context-specific hypotheses, several of which we experimentally validate. Furthermore, the data provide a unique resource for computational methods development, permitting empirical assessment of causal network learning in a complex, mammalian setting.
Pubmed ID: 28017544 RIS Download
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Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsCommercial antibody vendor which supplies antibodies and other products to life science researchers.
View all literature mentionsCommercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.
View all literature mentionsA contract research organization providing drug development and animal testing services. Under the name Covance Research Products Inc., based in Denver, Pennsylvania, the company also deals in the import, breeding and sale of laboratory animals. It breeds dogs, rabbits, guinea pigs, non-human primates, and pigs, and runs the largest non-human primate laboratory in Germany. (Wikipedia)
View all literature mentionsAutomated software program for image analysis based on microarray based image application. Various output styles are available, including image overlay with warping and registration and scattered correlation plot with 2D or 3D spot profile. Outputs can be formatted in text, xml, excel, or custom format.
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Hsp90
View all literature mentionsThis monoclonal targets alpha Tubulin
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis monoclonal targets Ybx1
View all literature mentionsThis polyclonal targets Human YB-1, aa 1-55
View all literature mentionsThis polyclonal targets YAP1
View all literature mentionsThis polyclonal targets YAP (H-125)
View all literature mentionsThis polyclonal targets XRCC1
View all literature mentionsThis monoclonal targets VHL
View all literature mentionsThis monoclonal targets VEGF Receptor 2 (55B11) Rabbit mAb
View all literature mentionsThis monoclonal targets Tsc2
View all literature mentionsThis unknown targets Transferrin Receptor
View all literature mentionsThis monoclonal targets Transglutaminase II, Cellular / Tissue
View all literature mentionsThis polyclonal targets TIGAR antibody
View all literature mentionsThis polyclonal targets WWTR1
View all literature mentionsThis polyclonal targets TAZ
View all literature mentionsThis monoclonal targets Tau
View all literature mentionsThis monoclonal targets Human SYK
View all literature mentionsThis monoclonal targets Stat5a
View all literature mentionsThis polyclonal targets Phospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at Tyr705. The antibody does not cross-react with other Stat proteins when phosphorylated on the corresponding tyrosine residue. Does cross-react withPhospho-EGFR
View all literature mentionsThis polyclonal targets Phospho-Src (Tyr527)
View all literature mentionsThis polyclonal targets Phospho-Src Family (Tyr416)
View all literature mentionsThis monoclonal targets Src
View all literature mentionsThis monoclonal targets SNAI1
View all literature mentionsThis monoclonal targets Raised against amino acids 1-552 representing full length Smad4 of human origin
View all literature mentionsThis monoclonal targets SRSF1
View all literature mentionsThis monoclonal targets SCD (CD.E10)
View all literature mentionsThis polyclonal targets Phospho-S6 Ribosomal Protein (Ser240/244)
View all literature mentionsThis polyclonal targets Phospho-S6 Ribosomal Protein (Ser235/236)
View all literature mentionsThis monoclonal targets Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-Rb (Ser807/811)
View all literature mentionsThis monoclonal targets Rb (4H1) Mouse mAb
View all literature mentionsThis monoclonal targets Rad50
View all literature mentionsThis polyclonal targets RAB11A
View all literature mentionsThis polyclonal targets PTCH, aa 1214-1312
View all literature mentionsThis unknown targets Phospho-PRAS40 (Thr246)
View all literature mentionsThis monoclonal targets PGR
View all literature mentionsThis polyclonal targets Phospho-PKC (pan) (βII Ser660)
View all literature mentionsThis polyclonal targets phospho-PKCdelta (Ser664)
View all literature mentionsThis polyclonal targets PKC alpha, phospho (Ser657)
View all literature mentionsThis monoclonal targets Prkca
View all literature mentionsThis polyclonal targets PI3 Kinase p85
View all literature mentionsThis unknown targets Phospho-PEA15 (Ser116)
View all literature mentionsThis polyclonal targets Pea15a
View all literature mentionsThis monoclonal targets PCNA
View all literature mentionsThis monoclonal targets Pxn
View all literature mentionsThis monoclonal targets PARP1
View all literature mentionsThis unknown targets p90RSK, phospho (Thr359 / Ser363)
View all literature mentionsThis monoclonal targets RPS6KB1
View all literature mentionsThis polyclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis polyclonal targets p38 MAPK
View all literature mentionsThis polyclonal targets p27 KIP 1 (phospho T198)
View all literature mentionsThis polyclonal targets Phospho-p27/Kip1 (T157) Affinity Purified Ab
View all literature mentionsThis monoclonal targets PSMD9
View all literature mentionsThis unknown targets Raised against a peptide mapping at the carboxy terminus of human p21
View all literature mentionsThis polyclonal targets NOTCH3
View all literature mentionsThis monoclonal targets Human Notch1
View all literature mentionsThis monoclonal targets NF-KappaB p65, phospho (Ser536)
View all literature mentionsThis polyclonal targets NDRG1
View all literature mentionsThis polyclonal targets N-Cadherin
View all literature mentionsThis unknown targets Myosin heavy chain 11
View all literature mentionsThis polyclonal targets Phospho-mTOR (Ser2448)
View all literature mentionsThis monoclonal targets mTOR
View all literature mentionsThis monoclonal targets Human MSH2
View all literature mentionsThis monoclonal targets Mre11 (31H4) Rabbit mAb
View all literature mentionsThis monoclonal targets Human ERRFI1
View all literature mentionsThis monoclonal targets MGMT (O6-methylguanine-DNA methyltransferase)
View all literature mentionsThis monoclonal targets Phospho-MEK1/2 (Ser217/221)
View all literature mentionsThis monoclonal targets MEK1, C-terminus
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-SAPK/JNK (Thr183/Tyr185)
View all literature mentionsThis polyclonal targets INPP4B
View all literature mentionsThis polyclonal targets Igf1r
View all literature mentionsThis polyclonal targets phospho-erbB-2/HER-2 (Tyr1248)
View all literature mentionsThis monoclonal targets c-erbB-2 / HER-2 / neu Oncoprotein
View all literature mentionsThis polyclonal targets Phospho-GSK-3 (Ser9)
View all literature mentionsThis polyclonal targets Phospho-GSK-3alpha/beta (Ser21/9)
View all literature mentionsThis monoclonal targets GSK3B
View all literature mentionsThis monoclonal targets GATA3
View all literature mentionsThis monoclonal targets Gab2
View all literature mentionsThis unknown targets FKHRL1, phospho (Ser318 / Ser321)
View all literature mentionsThis monoclonal targets FoxO3a
View all literature mentionsThis monoclonal targets FoxM1 (D12D5) XP Rabbit mAb
View all literature mentionsThis monoclonal targets Human ERCC1
View all literature mentionsThis monoclonal targets Human ER alpha, phospho (Ser118)
View all literature mentionsThis monoclonal targets Estrogen Receptor
View all literature mentionsThis polyclonal targets eIF4E
View all literature mentionsThis monoclonal targets Human EGF Receptor, phospho (Tyr1173)
View all literature mentionsThis polyclonal targets Phospho-EGF Receptor (Tyr1068)
View all literature mentionsThis polyclonal targets EGFR (1005)
View all literature mentionsThis polyclonal targets Cdh1
View all literature mentionsThis monoclonal targets PARK7/DJ1
View all literature mentionsThis monoclonal targets cyclin E (HE12)
View all literature mentionsThis polyclonal targets CCND1, CCND2
View all literature mentionsThis monoclonal targets CCNB1
View all literature mentionsThis polyclonal targets COL6A1
View all literature mentionsThis unknown targets Claudin 7
View all literature mentionsThis polyclonal targets HIAP-2 (cIAP-1)
View all literature mentionsThis monoclonal targets CHEK2
View all literature mentionsThis monoclonal targets Chk2
View all literature mentionsThis monoclonal targets CD49b
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis unknown targets Human Caspase 9, cleaved (Asp330)
View all literature mentionsThis polyclonal targets Cleaved Caspase-7 (Asp198)
View all literature mentionsThis monoclonal targets Casp3
View all literature mentionsThis monoclonal targets c-Raf, phospho (Ser338)
View all literature mentionsThis polyclonal targets MYC
View all literature mentionsThis monoclonal targets Raf-1
View all literature mentionsThis monoclonal targets c-Kit antibody [Y145]
View all literature mentionsThis monoclonal targets Human Bim
View all literature mentionsThis monoclonal targets Bcl-x
View all literature mentionsThis monoclonal targets BCL2 oncoprotein
View all literature mentionsThis monoclonal targets Human Bak
View all literature mentionsThis monoclonal targets Raf-B (F-7)
View all literature mentionsThis monoclonal targets Annexin VII
View all literature mentionsThis unknown targets Annexin A1
View all literature mentionsThis polyclonal targets AMPK-alpha
View all literature mentionsThis monoclonal targets CAMKV
View all literature mentionsThis monoclonal targets Akt (pan) (C67E7) Rabbit mAb
View all literature mentionsThis monoclonal targets Acaca
View all literature mentionsThis polyclonal targets YWHAB
View all literature mentionsThis polyclonal targets
View all literature mentionsThis polyclonal targets Phospho-p70 S6 Kinase (Thr389)
View all literature mentionsThis polyclonal targets β-Catenin
View all literature mentionsThis unknown targets Bcl-xL
View all literature mentionsThis monoclonal targets AMPK-alpha, phospho (Thr172)
View all literature mentionsThis polyclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis polyclonal targets Phospho-Acetyl-CoA Carboxylase (Ser79)
View all literature mentionsThis unknown targets 53BP1
View all literature mentionsThis monoclonal targets Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
View all literature mentionsThis polyclonal targets 4E-BP1
View all literature mentionsThis polyclonal targets YWHAZ, YWHAB, SFN
View all literature mentionsThis monoclonal targets 14-3-3 epsilon (8C3)
View all literature mentionsCell line BT-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-20 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line UACC-812 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MCF-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsSoftware platform for complex network analysis and visualization. Used for visualization of molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data.
View all literature mentionsMulti paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets Phospho-mTOR (Ser2448)
View all literature mentionsThis polyclonal targets Phospho-GSK-3alpha/beta (Ser21/9)
View all literature mentionsThis unknown targets Annexin A1
View all literature mentionsCell line BT-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-20 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line UACC-812 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MCF-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis monoclonal targets Hsp90
View all literature mentionsThis monoclonal targets alpha Tubulin
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis monoclonal targets Ybx1
View all literature mentionsThis polyclonal targets Human YB-1, aa 1-55
View all literature mentionsThis polyclonal targets YAP1
View all literature mentionsThis polyclonal targets YAP (H-125)
View all literature mentionsThis polyclonal targets XRCC1
View all literature mentionsThis monoclonal targets VHL
View all literature mentionsThis monoclonal targets VEGF Receptor 2 (55B11) Rabbit mAb
View all literature mentionsThis monoclonal targets Tsc2
View all literature mentionsThis unknown targets Transferrin Receptor
View all literature mentionsThis monoclonal targets Transglutaminase II, Cellular / Tissue
View all literature mentionsThis polyclonal targets TIGAR antibody
View all literature mentionsThis polyclonal targets WWTR1
View all literature mentionsThis polyclonal targets TAZ
View all literature mentionsThis monoclonal targets Tau
View all literature mentionsThis monoclonal targets Human SYK
View all literature mentionsThis monoclonal targets Stat5a
View all literature mentionsThis polyclonal targets Phospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at Tyr705. The antibody does not cross-react with other Stat proteins when phosphorylated on the corresponding tyrosine residue. Does cross-react withPhospho-EGFR
View all literature mentionsThis polyclonal targets Phospho-Src (Tyr527)
View all literature mentionsThis polyclonal targets Phospho-Src Family (Tyr416)
View all literature mentionsThis monoclonal targets Src
View all literature mentionsThis monoclonal targets SNAI1
View all literature mentionsThis monoclonal targets Raised against amino acids 1-552 representing full length Smad4 of human origin
View all literature mentionsThis monoclonal targets SRSF1
View all literature mentionsThis monoclonal targets SCD (CD.E10)
View all literature mentionsThis polyclonal targets Phospho-S6 Ribosomal Protein (Ser240/244)
View all literature mentionsThis polyclonal targets Phospho-S6 Ribosomal Protein (Ser235/236)
View all literature mentionsThis monoclonal targets Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-Rb (Ser807/811)
View all literature mentionsThis monoclonal targets Rb (4H1) Mouse mAb
View all literature mentionsThis monoclonal targets Rad50
View all literature mentionsThis polyclonal targets RAB11A
View all literature mentionsThis polyclonal targets PTCH, aa 1214-1312
View all literature mentionsThis unknown targets Phospho-PRAS40 (Thr246)
View all literature mentionsThis monoclonal targets PGR
View all literature mentionsThis polyclonal targets Phospho-PKC (pan) (βII Ser660)
View all literature mentionsThis polyclonal targets phospho-PKCdelta (Ser664)
View all literature mentionsThis polyclonal targets PKC alpha, phospho (Ser657)
View all literature mentionsThis monoclonal targets Prkca
View all literature mentionsThis polyclonal targets PI3 Kinase p85
View all literature mentionsThis unknown targets Phospho-PEA15 (Ser116)
View all literature mentionsThis polyclonal targets Pea15a
View all literature mentionsThis monoclonal targets PCNA
View all literature mentionsThis monoclonal targets Pxn
View all literature mentionsThis monoclonal targets PARP1
View all literature mentionsThis unknown targets p90RSK, phospho (Thr359 / Ser363)
View all literature mentionsThis monoclonal targets RPS6KB1
View all literature mentionsThis polyclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis polyclonal targets p38 MAPK
View all literature mentionsThis polyclonal targets p27 KIP 1 (phospho T198)
View all literature mentionsThis polyclonal targets Phospho-p27/Kip1 (T157) Affinity Purified Ab
View all literature mentionsThis monoclonal targets PSMD9
View all literature mentionsThis unknown targets Raised against a peptide mapping at the carboxy terminus of human p21
View all literature mentionsThis polyclonal targets NOTCH3
View all literature mentionsThis monoclonal targets Human Notch1
View all literature mentionsThis monoclonal targets NF-KappaB p65, phospho (Ser536)
View all literature mentionsThis polyclonal targets NDRG1
View all literature mentionsThis polyclonal targets N-Cadherin
View all literature mentionsThis unknown targets Myosin heavy chain 11
View all literature mentionsThis monoclonal targets mTOR
View all literature mentionsThis monoclonal targets Human MSH2
View all literature mentionsThis monoclonal targets Mre11 (31H4) Rabbit mAb
View all literature mentionsThis monoclonal targets Human ERRFI1
View all literature mentionsThis monoclonal targets MGMT (O6-methylguanine-DNA methyltransferase)
View all literature mentionsThis monoclonal targets Phospho-MEK1/2 (Ser217/221)
View all literature mentionsThis monoclonal targets MEK1, C-terminus
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-SAPK/JNK (Thr183/Tyr185)
View all literature mentionsThis polyclonal targets INPP4B
View all literature mentionsThis polyclonal targets Igf1r
View all literature mentionsThis polyclonal targets phospho-erbB-2/HER-2 (Tyr1248)
View all literature mentionsThis monoclonal targets c-erbB-2 / HER-2 / neu Oncoprotein
View all literature mentionsThis polyclonal targets Phospho-GSK-3 (Ser9)
View all literature mentionsThis monoclonal targets GSK3B
View all literature mentionsThis monoclonal targets GATA3
View all literature mentionsThis monoclonal targets Gab2
View all literature mentionsThis unknown targets FKHRL1, phospho (Ser318 / Ser321)
View all literature mentionsThis monoclonal targets FoxO3a
View all literature mentionsThis monoclonal targets FoxM1 (D12D5) XP Rabbit mAb
View all literature mentionsThis monoclonal targets Human ERCC1
View all literature mentionsThis monoclonal targets Human ER alpha, phospho (Ser118)
View all literature mentionsThis monoclonal targets Estrogen Receptor
View all literature mentionsThis polyclonal targets eIF4E
View all literature mentionsThis monoclonal targets Human EGF Receptor, phospho (Tyr1173)
View all literature mentionsThis polyclonal targets Phospho-EGF Receptor (Tyr1068)
View all literature mentionsThis polyclonal targets EGFR (1005)
View all literature mentionsThis polyclonal targets Cdh1
View all literature mentionsThis monoclonal targets PARK7/DJ1
View all literature mentionsThis monoclonal targets cyclin E (HE12)
View all literature mentionsThis polyclonal targets CCND1, CCND2
View all literature mentionsThis monoclonal targets CCNB1
View all literature mentionsThis polyclonal targets COL6A1
View all literature mentionsThis unknown targets Claudin 7
View all literature mentionsThis polyclonal targets HIAP-2 (cIAP-1)
View all literature mentionsThis monoclonal targets CHEK2
View all literature mentionsThis monoclonal targets Chk2
View all literature mentionsThis monoclonal targets CD49b
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis unknown targets Human Caspase 9, cleaved (Asp330)
View all literature mentionsThis polyclonal targets Cleaved Caspase-7 (Asp198)
View all literature mentionsThis monoclonal targets Casp3
View all literature mentionsThis monoclonal targets c-Raf, phospho (Ser338)
View all literature mentionsThis monoclonal targets Raf-1
View all literature mentionsThis polyclonal targets MYC
View all literature mentionsThis monoclonal targets c-Kit antibody [Y145]
View all literature mentionsThis monoclonal targets Human Bim
View all literature mentionsThis monoclonal targets Bcl-x
View all literature mentionsThis monoclonal targets BCL2 oncoprotein
View all literature mentionsThis monoclonal targets Human Bak
View all literature mentionsThis monoclonal targets Raf-B (F-7)
View all literature mentionsThis monoclonal targets Annexin VII
View all literature mentionsThis polyclonal targets AMPK-alpha
View all literature mentionsThis monoclonal targets CAMKV
View all literature mentionsThis monoclonal targets Akt (pan) (C67E7) Rabbit mAb
View all literature mentionsThis monoclonal targets Acaca
View all literature mentionsThis polyclonal targets YWHAB
View all literature mentionsThis unknown targets Bcl-xL
View all literature mentionsThis polyclonal targets β-Catenin
View all literature mentionsThis monoclonal targets AMPK-alpha, phospho (Thr172)
View all literature mentionsThis polyclonal targets Phospho-Acetyl-CoA Carboxylase (Ser79)
View all literature mentionsThis polyclonal targets
View all literature mentionsThis polyclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis unknown targets 53BP1
View all literature mentionsThis polyclonal targets 4E-BP1
View all literature mentionsThis polyclonal targets YWHAZ, YWHAB, SFN
View all literature mentionsThis monoclonal targets Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
View all literature mentionsThis monoclonal targets 14-3-3 epsilon (8C3)
View all literature mentionsThis monoclonal targets 14-3-3 epsilon (8C3)
View all literature mentionsThis polyclonal targets YWHAZ, YWHAB, SFN
View all literature mentionsThis polyclonal targets 4E-BP1
View all literature mentionsThis polyclonal targets
View all literature mentionsThis polyclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis polyclonal targets Phospho-Acetyl-CoA Carboxylase (Ser79)
View all literature mentionsThis monoclonal targets Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
View all literature mentionsThis unknown targets 53BP1
View all literature mentionsThis monoclonal targets AMPK-alpha, phospho (Thr172)
View all literature mentionsThis polyclonal targets β-Catenin
View all literature mentionsThis unknown targets Bcl-xL
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