Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

EGFR Dynamics Change during Activation in Native Membranes as Revealed by NMR.

Cell | 2016

The epidermal growth factor receptor (EGFR) represents one of the most common target proteins in anti-cancer therapy. To directly examine the structural and dynamical properties of EGFR activation by the epidermal growth factor (EGF) in native membranes, we have developed a solid-state nuclear magnetic resonance (ssNMR)-based approach supported by dynamic nuclear polarization (DNP). In contrast to previous crystallographic results, our experiments show that the ligand-free state of the extracellular domain (ECD) is highly dynamic, while the intracellular kinase domain (KD) is rigid. Ligand binding restricts the overall and local motion of EGFR domains, including the ECD and the C-terminal region. We propose that the reduction in conformational entropy of the ECD by ligand binding favors the cooperative binding required for receptor dimerization, causing allosteric activation of the intracellular tyrosine kinase.

Pubmed ID: 27839865 RIS Download

Mesh terms: Cell Line, Tumor | Epidermal Growth Factor | Humans | Intracellular Membranes | Nuclear Magnetic Resonance, Biomolecular | Protein Multimerization | Receptor, Epidermal Growth Factor | Thermodynamics | Transport Vesicles

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


IRDye 680RD Goat anti-Mouse IgG (H + L), 25 uL antibody (antibody)

RRID:AB_10956589

This unknown targets IRDye 680RD Goat anti-Mouse IgG (H + L) 25 uL

View all literature mentions

Claudin 4 Polyclonal Antibody (antibody)

RRID:AB_2336056

This monoclonal antibody targets Actin

View all literature mentions

NIH 3T3 (cell line)

RRID:CVCL_0594

Cell line NIH 3T3 is aSpontaneously immortalized cell line with a species of origin Mus musculus

View all literature mentions