To determine whether L-type voltage-operated Ca2+ channels (L-VOCCs) are required for oligodendrocyte progenitor cell (OPC) development, we generated an inducible conditional knock-out mouse in which the L-VOCC isoform Cav1.2 was postnatally deleted in NG2-positive OPCs. A significant hypomyelination was found in the brains of the Cav1.2 conditional knock-out (Cav1.2KO) mice specifically when the Cav1.2 deletion was induced in OPCs during the first 2 postnatal weeks. A decrease in myelin proteins expression was visible in several brain structures, including the corpus callosum, cortex, and striatum, and the corpus callosum of Cav1.2KO animals showed an important decrease in the percentage of myelinated axons and a substantial increase in the mean g-ratio of myelinated axons. The reduced myelination was accompanied by an important decline in the number of myelinating oligodendrocytes and in the rate of OPC proliferation. Furthermore, using a triple transgenic mouse in which all of the Cav1.2KO OPCs were tracked by a Cre reporter, we found that Cav1.2KO OPCs produce less mature oligodendrocytes than control cells. Finally, live-cell imaging in early postnatal brain slices revealed that the migration and proliferation of subventricular zone OPCs is decreased in the Cav1.2KO mice. These results indicate that the L-VOCC isoform Cav1.2 modulates oligodendrocyte development and suggest that Ca2+ influx mediated by L-VOCCs in OPCs is necessary for normal myelination.
Pubmed ID: 27798140 RIS Download
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This unknown targets Myelin Oligodendrocyte Glycoprotein (MOG)
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View all literature mentionsThis polyclonal targets Olig1
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View all literature mentionsMus musculus with name B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J from IMSR.
View all literature mentionsThis monoclonal targets Myelin Basic Protein
View all literature mentionsThis monoclonal targets Myelin Basic Protein
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View all literature mentionsThis monoclonal targets CD140a (PDGF Receptor alpha)
View all literature mentionsMus musculus with name B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J from IMSR.
View all literature mentionsThis unknown targets PLP1
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View all literature mentionsThis polyclonal targets Olig1
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View all literature mentionsThis monoclonal targets CD140a (PDGF Receptor alpha)
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View all literature mentionsImaging software used to acquire and analyze images from specific Bio-Rad imaging systems. Users can analyze gel or blot features, capture optimized image data, and generate a report of the data. Image Lab software exclusively runs on the Gel Doc EZ imager, Gel Doc XR+ imaging system, ChemiDo MP, ChemiDoc XRS+ imaging systems, Criterion Stain Free imager, and the GS-900calibrated densitometer.
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View all literature mentionsThis polyclonal targets Cleaved Caspase-3 (Asp175)
View all literature mentionsThis unknown targets Myelin Oligodendrocyte Glycoprotein (MOG)
View all literature mentionsThis unknown targets Myelin Oligodendrocyte Glycoprotein (MOG)
View all literature mentionsThis unknown targets Myelin Oligodendrocyte Glycoprotein (MOG)
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View all literature mentionsThis monoclonal targets APC
View all literature mentionsThis monoclonal targets epitope 264-216 of proteolipid protein
View all literature mentionsThis monoclonal targets epitope 264-216 of proteolipid protein
View all literature mentionsSoftware designed for single or multi-wavelength intracellular ion measurements. It provides simultaneous display of raw data, ratio image, graphs of intensities, ratios, ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities.
View all literature mentionsSoftware designed for single or multi-wavelength intracellular ion measurements. It provides simultaneous display of raw data, ratio image, graphs of intensities, ratios, ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities.
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View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsSoftware tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
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View all literature mentionsThis monoclonal targets Ptprc
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