Glutamate-ammonia ligase (GLUL) belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. Here, we found higher expression of GLUL in the breast cancer patients was associated with larger tumor size and higher level of HER2 expression. In addition, GLUL was heterogeneously expressed in various breast cancer cells. The mRNA and protein expression levels of GLUL in SK-BR-3 cells were obviously higher than that in the other types of breast cancer cells. Results showed GLUL knockdown in SK-BR-3 cells could significantly decrease the proliferation ability. Furthermore, GLUL knockdown markedly inhibited the p38 MAPK and ERK1/ERK2 signaling pathways in SK-BR-3 cells. Thus, GLUL may represent a novel target for selectively inhibiting p38 MAPK and ERK1/ERK2 signaling pathways and the proliferation potential of breast cancer cells. J. Cell. Biochem. 118: 2018-2025, 2017. © 2016 Wiley Periodicals, Inc.
Pubmed ID: 27791265 RIS Download
Mesh terms: Adult | Aged | Breast Neoplasms | Cell Line, Tumor | Cell Proliferation | Female | Gene Expression Regulation, Neoplastic | Glutamate-Ammonia Ligase | Humans | Middle Aged | Mitogen-Activated Protein Kinase 1 | Mitogen-Activated Protein Kinase 3 | Neoplasm Grading | Neoplasm Staging | RNA, Messenger | RNA, Small Interfering | Receptor, ErbB-2 | Signal Transduction | Survival Analysis | p38 Mitogen-Activated Protein Kinases
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