Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Publication

Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila.

Nidhi Sharma Dey | Parvathy Ramesh | Mayank Chugh | Sudip Mandal | Lolitika Mandal

eLife | 2016

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).

Pubmed ID: 27782877 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

Agency: Wellcome Trust, United Kingdom
Agency: DBT-Wellcome Trust India Alliance, India
Id: 500124-Z-09-Z
Agency: Wellcome Trust, United Kingdom
Id: 500124/Z09/Z

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Goat Anti-Mouse IgG (H+L) Highly Cross-adsorbed Antibody, Alexa Fluor ?? 647 Conjugated (antibody)

RRID:AB_2535805

This unknown targets IgG (H+L)

View all literature mentions

Goat Anti-Rat IgG (H+L) Antibody, Alexa Fluor ?? 647 Conjugated (antibody)

RRID:AB_141778

This polyclonal secondary targets IgG (H+L)

View all literature mentions

Alexa Fluor 647-AffiniPure Goat Anti-Mouse IgG (H+L) (antibody)

RRID:AB_2338902

This unknown targets Mouse IgG (H+L)

View all literature mentions

Alexa Fluor 647-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (antibody)

RRID:AB_2340693

This unknown targets Rat IgG (H+L)

View all literature mentions

Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (antibody)

RRID:AB_2307443

This polyclonal targets IgG (H+L)

View all literature mentions

Cy3-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Rat Sr Prot) (antibody)

RRID:AB_2338692

This unknown targets Mouse IgG (H+L)

View all literature mentions

Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_10562719

This polyclonal secondary targets Rat IgG (H+L)

View all literature mentions

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_2534078

This polyclonal secondary targets Rabbit IgG (H+L)

View all literature mentions

Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (antibody)

RRID:AB_2534072

This unknown targets IgG (H+L)

View all literature mentions

Fluorescein (FITC)-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) (antibody)

RRID:AB_2337978

This unknown targets Rabbit IgG (H+L)

View all literature mentions

Fluorescein (FITC)-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Rat Sr Prot) (antibody)

RRID:AB_2338601

This unknown targets Mouse IgG (H+L)

View all literature mentions

Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Biotinylated) (antibody)

RRID:AB_10694226

This monoclonal targets Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (ylated)

View all literature mentions

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

View all literature mentions

Nimrod C1 antibody (antibody)

RRID:AB_2568423

This monoclonal targets NimC1

View all literature mentions

Mouse Anti-Green Fluorescent Protein (GFP) Monoclonal Antibody, Unconjugated, Clone GFP-20 (antibody)

RRID:AB_259941

This monoclonal targets Green Fluorescent Protein (GFP)

View all literature mentions

Anti-??-Galactosidase Monoclonal Antibody, Unconjugated (antibody)

RRID:AB_430877

This monoclonal targets & x03B2;-Galactosidase

View all literature mentions

CD2 antibody (antibody)

RRID:AB_1283138

This monoclonal targets CD2 / LFA-2

View all literature mentions

Mouse Anti-Drosophila Trio Monoclonal Antibody, Unconjugated (antibody)

RRID:AB_528494

This monoclonal targets Mouse Drosophila Trio

View all literature mentions

hindsight: zinc-finger nuclear protein hindsight antibody - Lipshitz, H.D.; Hospital for Sick Children (antibody)

RRID:AB_528278

This monoclonal targets hindsight: zinc-finger nuclear protein hindsight

View all literature mentions

Mouse Anti-Drosophila Wingless protein Monoclonal Antibody, Unconjugated (antibody)

RRID:AB_528512

This monoclonal targets Mouse Drosophila Wingless protein

View all literature mentions

Cubitus interruptus (Ci) full length antibody - Holmgren, R.; Northwestern University (antibody)

RRID:AB_2109711

This monoclonal targets Cubitus interruptus (Ci) full length

View all literature mentions

Antennapedia protein antibody - Brower, D.; University of Arizona (antibody)

RRID:AB_528082

This monoclonal targets Antennapedia protein

View all literature mentions

Lamin C antibody - Fisher, P.A.; State University of New York at Stony Brook (antibody)

RRID:AB_528339

This monoclonal targets Lamin C

View all literature mentions

Adobe Photoshop (software resource)

RRID:SCR_002078

Software for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.

View all literature mentions

Imaris (software resource)

RRID:SCR_007370

Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.

View all literature mentions

ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions

Adobe Photoshop (software resource)

RRID:SCR_002078

Software for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.

View all literature mentions

Adobe Photoshop (software resource)

RRID:SCR_002078

Software for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.

View all literature mentions