Metabolic studies suggest that the absorptive capacity of the small intestine for fructose is limited, though the molecular mechanisms controlling this process remain unknown. Here we demonstrate that thioredoxin-interacting protein (Txnip), which regulates glucose homeostasis in mammals, binds to fructose transporters and promotes fructose absorption by the small intestine. Deletion of Txnip in mice reduced fructose transport into the peripheral bloodstream and liver, as well as the severity of adverse metabolic outcomes resulting from long-term fructose consumption. We also demonstrate that fructose consumption induces expression of Txnip in the small intestine. Diabetic mice had increased expression of Txnip in the small intestine as well as enhanced fructose uptake and transport into the hepatic portal circulation. The deletion of Txnip in mice abolished the diabetes-induced increase in fructose absorption. Our results indicate that Txnip is a critical regulator of fructose metabolism and suggest that a diabetic state can promote fructose uptake.
Pubmed ID: 27725089 RIS Download
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laboratory mouse with name C57BL/6 from MGI.
View all literature mentionsCell line Caco-2 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets SLC2A2
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets TXNIP
View all literature mentionsThis monoclonal targets Mouse rabbit IgG (Light-Chain Specific) (L57A3) mAb
View all literature mentionsThis polyclonal targets SLC2A5
View all literature mentionsThis unknown targets Goat Mouse IgG (H L)-HRP Conjugate
View all literature mentionsThis polyclonal targets SLC2A2
View all literature mentionsThis polyclonal targets V5 tag - ChIP Grade
View all literature mentionsThis polyclonal targets DDDDK tag
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsThis polyclonal targets SLC2A2
View all literature mentionsMus musculus with name B6;129-Txniptm1.1Rlee/J from IMSR.
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets TXNIP
View all literature mentionsThis unknown targets Goat Mouse IgG (H L)-HRP Conjugate
View all literature mentionsThis monoclonal targets Mouse rabbit IgG (Light-Chain Specific) (L57A3) mAb
View all literature mentionsThis polyclonal targets SLC2A5
View all literature mentionsThis polyclonal targets SLC2A2
View all literature mentionsThis polyclonal targets DDDDK tag
View all literature mentionsThis polyclonal targets V5 tag - ChIP Grade
View all literature mentionsMus musculus with name B6;129-Txniptm1.1Rlee/J from IMSR.
View all literature mentions