Much of the Hippo and planar cell polarity (PCP) signaling mediated by the Drosophila protocadherin Fat depends on its ability to change the subcellular localization, levels and activity of the unconventional myosin Dachs. To better understand this process, we have performed a structure-function analysis of Dachs, and used this to identify a novel and important mediator of Fat and Dachs activities, a Dachs-binding SH3 protein we have named Dlish. We found that Dlish is regulated by Fat and Dachs, that Dlish also binds Fat and the Dachs regulator Approximated, and that Dlish is required for Dachs localization, levels and activity in both wild type and fat mutant tissue. Our evidence supports dual roles for Dlish. Dlish tethers Dachs to the subapical cell cortex, an effect partly mediated by the palmitoyltransferase Approximated under the control of Fat. Conversely, Dlish promotes the Fat-mediated degradation of Dachs.
Pubmed ID: 27692068 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A commercial antibody supplier and provider of various services.
View all literature mentionsA commercial antibody vendor, specializing in secondary antibodies.
View all literature mentionsBiomaterial supply resource which collects, maintains, and distributes independent transgenic fly lines. Most of the 38,000 fly lines are RNAi lines, but VDRC also maintains a collection of enhancer-GAL4 driver lines. Nearly all lines are in duplicate. Users can search for the stocks or DNA constructs for the gene of interest by entering CG number, synonym, or Transformant ID.
View all literature mentionsCell line S2R+ is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsThis polyclonal targets FLAG
View all literature mentionsThis polyclonal targets V5 Tag
View all literature mentionsThis polyclonal secondary targets IgG, Light Chain Specific
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis monoclonal targets Cubitus interruptus (Ci) full length
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets GST (B-14)
View all literature mentionsThis polyclonal targets HA-probe
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis polyclonal targets E-cadherin (dP-20)
View all literature mentionsPublic image processing and analysis program for Macintosh.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsCell line S2R+-SQH-GFP is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsCell line CME W1 cl.8+ is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets GST (B-14)
View all literature mentionsThis polyclonal targets HA-probe
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsCell line S2R+-SQH-GFP is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsCell line CME W1 cl.8+ is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis polyclonal targets E-cadherin (dP-20)
View all literature mentionsThis monoclonal targets Cubitus interruptus (Ci) full length
View all literature mentionsThis polyclonal secondary targets IgG, Light Chain Specific
View all literature mentionsThis polyclonal targets FLAG
View all literature mentionsThis polyclonal targets V5 Tag
View all literature mentionsThis polyclonal secondary targets IgG, Light Chain Specific
View all literature mentionsThis polyclonal secondary targets IgG, Light Chain Specific
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis polyclonal targets V5 Tag
View all literature mentionsThis polyclonal targets FLAG
View all literature mentionsThis polyclonal targets FLAG
View all literature mentionsThis polyclonal targets V5 Tag
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis polyclonal targets FLAG
View all literature mentionsThis polyclonal targets V5 Tag
View all literature mentionsThis polyclonal secondary targets IgG, Light Chain Specific
View all literature mentions