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There has recently been a resurgence of interest in psychedelics, substances that profoundly alter perception and cognition and have recently demonstrated therapeutic efficacy to treat anxiety, depression, and addiction in the clinic. The receptor mechanisms that drive their molecular and behavioral effects involve activation of cortical serotonin 5-HT2A receptors, but the responses of specific cellular populations remain unknown. Here, we provide evidence that a small subset of 5-HT2A-expressing excitatory neurons is directly activated by psychedelics and subsequently recruits other select cell types including subpopulations of inhibitory somatostatin and parvalbumin GABAergic interneurons, as well as astrocytes, to produce distinct and regional responses. To gather data regarding the response of specific neuronal populations, we developed methodology for fluorescence-activated cell sorting (FACS) to segregate and enrich specific cellular subtypes in the brain. These methods allow for robust neuronal sorting based on cytoplasmic epitopes followed by downstream nucleic acid analysis, expanding the utility of FACS in neuroscience research.
Pubmed ID: 27649637 RIS Download
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This monoclonal targets c-Fos (C-10)
View all literature mentionsThis polyclonal targets SST
View all literature mentionsThis unknown targets 5-HT2A / HTR2A
View all literature mentionsThis monoclonal targets c-Fos
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG, Fc? Subclass 1 Specific
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal targets parvalbumin
View all literature mentionsThis monoclonal targets Milli-Mark NeuN-PE clone A60
View all literature mentionsThis monoclonal targets Phospho-c-Fos (Ser32) (D82C12) XP Rabbit mAb (Alexa Fluor 647 Conjugate)
View all literature mentionsThis unknown targets 5-HT2A / HTR2A
View all literature mentionsThis monoclonal targets c-Fos
View all literature mentionsThis unknown targets Mouse IgG, Fc? Subclass 1 Specific
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal targets parvalbumin
View all literature mentionsThis monoclonal targets Milli-Mark NeuN-PE clone A60
View all literature mentionsThis monoclonal targets Phospho-c-Fos (Ser32) (D82C12) XP Rabbit mAb (Alexa Fluor 647 Conjugate)
View all literature mentionsThis polyclonal targets SST
View all literature mentionsThis monoclonal targets c-Fos (C-10)
View all literature mentionsThis monoclonal targets c-Fos (C-10)
View all literature mentionsThis polyclonal targets SST
View all literature mentionsThis monoclonal targets c-Fos (C-10)
View all literature mentionsThis polyclonal targets SST
View all literature mentionsThis monoclonal targets c-Fos (C-10)
View all literature mentionsThis polyclonal targets SST
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