Aging is a major risk factor in many forms of late-onset neurodegenerative disorders. The ability to recapitulate age-related characteristics of human neurons in culture will offer unprecedented opportunities to study the biological processes underlying neuronal aging. Here, we show that using a recently demonstrated microRNA-based cellular reprogramming approach, human fibroblasts from postnatal to near centenarian donors can be efficiently converted into neurons that maintain multiple age-associated signatures. Application of an epigenetic biomarker of aging (referred to as epigenetic clock) to DNA methylation data revealed that the epigenetic ages of fibroblasts were highly correlated with corresponding age estimates of reprogrammed neurons. Transcriptome and microRNA profiles reveal genes differentially expressed between young and old neurons. Further analyses of oxidative stress, DNA damage and telomere length exhibit the retention of age-associated cellular properties in converted neurons from corresponding fibroblasts. Our results collectively demonstrate the maintenance of age after neuronal conversion.
Pubmed ID: 27644593 RIS Download
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This polyclonal targets MAP2 antibody - Neuronal Marker
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View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
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View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsAutomated benchtop flow cytometry system. It allows measuring four fluorochrome-conjugates, forward and side scatter.
View all literature mentionsSoftware for image capture, processing and analysis with Leica fluorescence and confocal microscopes.
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View all literature mentionsCell line AG08433 is a Finite cell line with a species of origin Homo sapiens (Human)
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View all literature mentionsCell line AG04379 is a Finite cell line with a species of origin Homo sapiens (Human)
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View all literature mentionsCell line AG04148 is a Finite cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line AG04060 is a Finite cell line with a species of origin Homo sapiens (Human)
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View all literature mentionsCell line AG07095 is a Finite cell line with a species of origin Homo sapiens (Human)
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