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Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.

Cell | Aug 25, 2016

Cellular compartments that cannot be biochemically isolated are challenging to characterize. Here we demonstrate the proteomic characterization of the synaptic clefts that exist at both excitatory and inhibitory synapses. Normal brain function relies on the careful balance of these opposing neural connections, and understanding how this balance is achieved relies on knowledge of their protein compositions. Using a spatially restricted enzymatic tagging strategy, we mapped the proteomes of two of the most common excitatory and inhibitory synaptic clefts in living neurons. These proteomes reveal dozens of synaptic candidates and assign numerous known synaptic proteins to a specific cleft type. The molecular differentiation of each cleft allowed us to identify Mdga2 as a potential specificity factor influencing Neuroligin-2's recruitment of presynaptic neurotransmitters at inhibitory synapses.

Pubmed ID: 27565350 RIS Download

Mesh terms: Animals | Antigens, CD | Cell Adhesion Molecules, Neuronal | GABAergic Neurons | Glutamic Acid | HEK293 Cells | Humans | Immunoglobulins | Membrane Glycoproteins | Mice | Nerve Tissue Proteins | Neural Cell Adhesion Molecules | Peroxidase | Proteome | Proteomics | Rats | Receptors, GABA | Recombinant Fusion Proteins | Synaptic Membranes | Thalamus

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Associated grants

  • Agency: , Id: R01 CA186568
  • Agency: NIGMS NIH HHS, Id: P41 GM103412
  • Agency: , Id: P41GM103412
  • Agency: NCI NIH HHS, Id: R01 CA186568
  • Agency: , Id: R01 NS092578
  • Agency: , Id: R01GM086197
  • Agency: , Id: R01 NS071008 01A1
  • Agency: NINDS NIH HHS, Id: R01 NS071008
  • Agency: HHMI, Id: R01 NS092578
  • Agency: NINDS NIH HHS, Id: R01 GM086197
  • Agency: NIGMS NIH HHS, Id:

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