Arterial occlusive diseases are major causes of morbidity and mortality. Blood flow to the affected tissue must be restored quickly if viability and function are to be preserved. We report that disruption of the mixed-lineage protein kinase (MLK) - cJun NH2-terminal kinase (JNK) signaling pathway in endothelial cells causes severe blockade of blood flow and failure to recover in the murine femoral artery ligation model of hindlimb ischemia. We show that the MLK-JNK pathway is required for the formation of native collateral arteries that can restore circulation following arterial occlusion. Disruption of the MLK-JNK pathway causes decreased Dll4/Notch signaling, excessive sprouting angiogenesis, and defects in developmental vascular morphogenesis. Our analysis demonstrates that the MLK-JNK signaling pathway is a key regulatory mechanism that protects against ischemia in arterial occlusive disease.
Pubmed ID: 27504807 RIS Download
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Commercial antibody supplier and developer for biomedical research. These products are compatible with use in flow cytometry and mass cytometry, immunoprecipitation and chip, western blotting, immunofluorescence microscopy, and quantitative multiplexing.
View all literature mentionsSoftware that estimates expression at transcript-level resolution and controls for variability evident across replicate libraries.
View all literature mentionsSoftware tool for transcriptome assembly and differential expression analysis for RNA-Seq. Includes script called cuffmerge that can be used to merge together several Cufflinks assemblies. It also handles running Cuffcompare as well as automatically filtering a number of transfrags that are likely to be artifacts. If the researcher has a reference GTF file, the researcher can provide it to the script to more effectively merge novel isoforms and maximize overall assembly quality.
View all literature mentionsThe main purpose of Cufflinks.cuffmerge is to merge together several Cufflinks assemblies, making it easier to produce an assembly GTF file suitable for use with Cufflinks.cuffdiff. Cufflinks.cuffmerge also runs Cuffcompare in the background and automatically filters out transcribed fragments (transfrags) that are likely to be artifacts. Trapnell C, Hendrickson D,Sauvageau S, Goff L, Rinn JL, Pachter L. Differential analysis of gene regulation at transcript resolution with RNA-seq. Nature Biotechnology. 2013;31:46-53.
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View all literature mentionsThis monoclonal targets CD144
View all literature mentionsThis monoclonal targets JUND
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis monoclonal targets Actin, α-Smooth Muscle
View all literature mentionsThis monoclonal targets NOTCH1
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets JNK1 / JNK2
View all literature mentionsThis unknown targets c-Jun, phospho (Ser63) II
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View all literature mentionsThis unknown targets Mouse CD19
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets CD45.1
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View all literature mentionsThis monoclonal targets CD102
View all literature mentionsThis monoclonal targets Actin, alpha, Smooth Muscle
View all literature mentionsThis polyclonal targets NG2 Chondroitin Sulfate Proteoglycan
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View all literature mentionsThis unknown targets Mouse Endomucin
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsCell line B16-F10 is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsAllele Detail: Targeted This is a legacy resource.
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View all literature mentionsMus musculus with name B6.129S2-Mapk9tm1Flv/J from IMSR.
View all literature mentionsMus musculus with name B6.129S1-Mapk8tm1Flv/J from IMSR.
View all literature mentionsMus musculus with name B6.CBA-Tg(Cdh5-cre/ERT2)1Rha from IMSR.
View all literature mentionsMus musculus with name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato.-EGFP)Luo/J from IMSR.
View all literature mentionsMus musculus with name B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J from IMSR.
View all literature mentionsMus musculus with name B6.FVB-Tg(Cdh5-cre)7Mlia/J from IMSR.
View all literature mentionsMus musculus with name B6.129P2-Lyz2tm1(cre)Ifo/J from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis monoclonal targets Human/Mouse DLL4 MAb (Clone 207822)
View all literature mentionsThis monoclonal targets Actin, α-Smooth Muscle
View all literature mentionsThis monoclonal targets JUND
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis monoclonal targets Human/Mouse DLL4 MAb (Clone 207822)
View all literature mentionsThis monoclonal targets CD144
View all literature mentionsThis monoclonal targets Human/Mouse DLL4 MAb (Clone 207822)
View all literature mentionsThis monoclonal targets CD144
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis monoclonal targets Human/Mouse DLL4 MAb (Clone 207822)
View all literature mentionsThis monoclonal targets Actin, α-Smooth Muscle
View all literature mentionsThis monoclonal targets Actin, α-Smooth Muscle
View all literature mentions