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Formation of polarity convergences underlying shoot outgrowths.

eLife | 2016

The development of outgrowths from plant shoots depends on formation of epidermal sites of cell polarity convergence with high intracellular auxin at their centre. A parsimonious model for generation of convergence sites is that cell polarity for the auxin transporter PIN1 orients up auxin gradients, as this spontaneously generates convergent alignments. Here we test predictions of this and other models for the patterns of auxin biosynthesis and import. Live imaging of outgrowths from kanadi1 kanadi2 Arabidopsis mutant leaves shows that they arise by formation of PIN1 convergence sites within a proximodistal polarity field. PIN1 polarities are oriented away from regions of high auxin biosynthesis enzyme expression, and towards regions of high auxin importer expression. Both expression patterns are required for normal outgrowth emergence, and may form part of a common module underlying shoot outgrowths. These findings are more consistent with models that spontaneously generate tandem rather than convergent alignments.

Pubmed ID: 27478985 RIS Download

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Antibodies used in this publication

Associated grants

  • Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
    Id: BBS/E/J/00000152
  • Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
    Id: BBS/E/J/000C0645
  • Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
    Id: BB/F005997/1
  • Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
    Id: BB/L008920/1

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PIN1 (aP-20) (antibody)

RRID:AB_670528

This polyclonal targets PIN1 (aP-20)

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VolView (software resource)

RRID:SCR_014569

A software for volume visualization that can be used by researchers to explore and analyze medical and scientific data. This software uses a variety of tools to load and visualize the data on either a 2D or 3D display. Theses tools include volume rendering, maximum intensity projections and oblique reformatting. Visualizations can be saved mid-session and be reopened at a later time.

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ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

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