We sought to determine the contribution of scaffold topography to the migration and morphology of neural stem cells by mimicking anatomical features of scaffolds found in vivo. We mimicked two types of central nervous system scaffolds encountered by neural stem cells during development in vitro by constructing different diameter electrospun polycaprolactone (PCL) fiber mats, a substrate that we have shown to be topographically similar to brain scaffolds. We compared the effects of large fibers (made to mimic blood vessel topography) with those of small-diameter fibers (made to mimic radial glial process topography) on the migration and differentiation of neural stem cells. Neural stem cells showed differential migratory and morphological reactions with laminin in different topographical contexts. We demonstrate, for the first time, that neural stem cell biological responses to laminin are dependent on topographical context. Large-fiber topography without laminin prevented cell migration, which was partially reversed by treatment with rock inhibitor. Cell morphology complexity assayed by fractal dimension was inhibited in nocodazole- and cytochalasin-D-treated neural precursor cells in large-fiber topography, but was not changed in small-fiber topography with these inhibitors. These data indicate that cell morphology has different requirements on cytoskeletal proteins dependent on the topographical environment encountered by the cell. We propose that the physical structure of distinct scaffolds induces unique signaling cascades that regulate migration and morphology in embryonic neural precursor cells. J. Comp. Neurol. 524:3485-3502, 2016. © 2016 Wiley Periodicals, Inc.
Pubmed ID: 27418162 RIS Download
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This unknown targets The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
View all literature mentionsThis monoclonal targets Recombinant protein corresponding to AA 1158-1345 of mouse origin
View all literature mentionsThis polyclonal targets Doublecortin
View all literature mentionsThis unknown targets Mouse Rat radial glial cell marker
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
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View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
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View all literature mentionsStatistical software that uses dynamic graphics rather than tables or graphs to visualize raw data. More specific versions of JMP are available for statistical analyses, clinical work, and genomics. Features include statistical modeling, data cleanup, automation and scripting, and experimental design.
View all literature mentionsThis monoclonal targets Recombinant protein corresponding to AA 1158-1345 of mouse origin
View all literature mentionsThis unknown targets The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsThis polyclonal targets Doublecortin
View all literature mentionsThis unknown targets The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
View all literature mentionsThis monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis unknown targets The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
View all literature mentions