The disruption of protein quality control networks is central to pathology in Huntington's disease (HD) and other neurodegenerative disorders. The aberrant accumulation of insoluble high-molecular-weight protein complexes containing the Huntingtin (HTT) protein and SUMOylated protein corresponds to disease manifestation. We previously identified an HTT-selective E3 SUMO ligase, PIAS1, that regulates HTT accumulation and SUMO modification in cells. Here we investigated whether PIAS1 modulation in neurons alters HD-associated phenotypes in vivo. Instrastriatal injection of a PIAS1-directed miRNA significantly improved behavioral phenotypes in rapidly progressing mutant HTT (mHTT) fragment R6/2 mice. PIAS1 reduction prevented the accumulation of mHTT and SUMO- and ubiquitin-modified proteins, increased synaptophysin levels, and normalized key inflammatory markers. In contrast, PIAS1 overexpression exacerbated mHTT-associated phenotypes and aberrant protein accumulation. These results confirm the association between aberrant accumulation of expanded polyglutamine-dependent insoluble protein species and pathogenesis, and they link phenotypic benefit to reduction of these species through PIAS1 modulation.
Pubmed ID: 27146268 RIS Download
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This polyclonal targets Tubulin, beta, Class III, Neuronal
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets PIAS1
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis monoclonal targets GAPDH - Loading Control
View all literature mentionsThis polyclonal targets Histone H3
View all literature mentionsThis monoclonal targets Genetic locus: RELA (human) mapping to 11q13.1; Rela (mouse) mapping to 19 A.
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets SUMO-2 / 3
View all literature mentionsThis polyclonal targets SUMO-1 (human) (CT) pAb
View all literature mentionsThis polyclonal targets Peptide derived from human huntingtin protein (a.a. 2-17)
View all literature mentionsThis monoclonal targets Huntingtin a.a. 1-82
View all literature mentionsThis unknown targets Chicken IgG
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis unknown targets The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
View all literature mentionsThis polyclonal targets Tubulin, beta, Class III, Neuronal
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets PIAS1
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE, documented on July 17, 2013. Commercial software vendor.
View all literature mentionsESCI (pronounced ''esky'') is a set of interactive simulations that run under Microsoft Excel. The set of Excel files lets students explore some key concepts in statistical and quantitative reasoning, including effect sizes, confidence intervals, and meta analysis. These are key concepts in the neuroscience toolkit, but can be particularly difficult to foster in undergraduate and graduate students. With ESCI you can: * explore many Confidence Interval (CI) concepts * calculate and display CIs for your own data, for some simple designs * calculate CIs for Cohen''s standardized effect size d * explore noncentral t distributions and their role in statistical power * use CIs for simple meta-analysis, using original or standardized units * explore all these concepts via vivid interactive graphical simulations. The simulations are free, excellent, and come with detailed supporting materials. Cumming also has a printed textbook companion which is quite good. Cumming, G. (2012). Understanding The New Statistics: Effect Sizes, Confidence Intervals, and Meta-Analysis. New York: Routledge * Explains estimation, with many examples. * Designed for any discipline that uses statistical significance testing. * For advanced undergraduate and graduate students, and researchers. * Comes with free ESCI software. * May be the first evidence-based statistics textbook. * Assumes only prior completion of any intro statistics course. * See the dance of the confidence intervals, and many other intriguing things. The Excel files truly stand on their own, though, with good feedback from their use in a basic stats class for psych and neuroscience majors. Finally, if you are like me and learned the ''old school'' statistics of null-hypothesis testing, here''s a great article by Gerd Gigerenzer to kick you into the ''new school'' of confidence intervals and effect sizes. Learn the dirty secrets of null hypothesis testing: Gigerenzer G, Kraus S & Vitouch O. (2004). The null ritual: What you always wanted to know about significance testing but were afraid to ask. The Sage Handbook of Quantitative Methodology for the Social Sciences, p. 391-408: http://library.mpib-berlin.mpg.de/ft/gg/GG_Null_2004.pdf
View all literature mentionsMus musculus with name B6CBA-Tg(HDexon1)62Gpb/3J from IMSR.
View all literature mentionsThis polyclonal targets Peptide derived from human huntingtin protein (a.a. 2-17)
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis monoclonal targets GAPDH - Loading Control
View all literature mentionsThis monoclonal targets Genetic locus: RELA (human) mapping to 11q13.1; Rela (mouse) mapping to 19 A.
View all literature mentionsThis polyclonal targets SUMO-1 (human) (CT) pAb
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets SUMO-2 / 3
View all literature mentionsThis polyclonal targets Histone H3
View all literature mentionsThis polyclonal targets Peptide derived from human huntingtin protein (a.a. 2-17)
View all literature mentionsThis monoclonal targets Huntingtin a.a. 1-82
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis unknown targets Chicken IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets Tubulin, beta, Class III, Neuronal
View all literature mentionsThis monoclonal targets PIAS1
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Tubulin, beta, Class III, Neuronal
View all literature mentionsThis monoclonal targets PIAS1
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
View all literature mentionsMus musculus with name B6CBA-Tg(HDexon1)62Gpb/3J from IMSR.
View all literature mentions