Searching across hundreds of databases
Fused in Sarcoma (FUS) is a multifunctional RNA-/DNA-binding protein, which is involved in the pathogenesis of the neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). A common hallmark of these disorders is the abnormal accumulation of mutated FUS protein in the cytoplasm. Under normal conditions FUS is confined to the nuclear compartment, in neurons, however, additional somatodendritic localization can be observed. In this study, we carefully analyzed the subcellular localization of endogenous FUS at synaptic sites of hippocampal neurons which are among the most affected cell types in FTD with FUS pathology. We could confirm a strong nuclear localization of FUS as well as its prominent and widespread neuronal expression throughout the adult and developing rat brain, particularly in the hippocampus, the cerebellum and the outer layers of the cortex. Intriguingly, FUS was also consistently observed at synaptic sites as detected by neuronal subcellular fractionation as well as by immunolabeling. To define a pre- and/or postsynaptic localization of FUS, we employed super-resolution fluorescence localization microscopy. FUS was found to be localized within the axon terminal in close proximity to the presynaptic vesicle protein Synaptophysin1 and adjacent to the active zone protein Bassoon, but well separated from the postsynaptic protein PSD-95. Having shown the presynaptic localization of FUS in the nervous system, a novel extranuclear role of FUS at neuronal contact sites has to be considered. Since there is growing evidence that local presynaptic translation might also be an important mechanism for plasticity, FUS - like the fragile X mental retardation protein FMRP - might act as one of the presynaptic RNA-binding proteins regulating this machinery. Our observation of presynaptic FUS should foster further investigations to determine its role in neurodegenerative diseases such as ALS and FTD.
Pubmed ID: 26834559 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Cell line NIH 3T3 is a Spontaneously immortalized cell line with a species of origin Mus musculus
View all literature mentionsThis polyclonal targets Homer 1b/c
View all literature mentionsThis monoclonal targets MAP2
View all literature mentionsThis polyclonal targets VGLUT 1
View all literature mentionsThis monoclonal targets S-100 (beta-Subunit)
View all literature mentionsThis polyclonal targets Synaptophysin 1
View all literature mentionsThis polyclonal targets Synaptophysin antibody
View all literature mentionsThis monoclonal targets PSD 95 (PDZ domain)
View all literature mentionsThis monoclonal targets PSD95 antibody [6G6-1C9]
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets Gephyrin
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets GAD65
View all literature mentionsThis polyclonal targets FUS
View all literature mentionsThis monoclonal targets FUS/TLS (4H11)
View all literature mentionsThis polyclonal targets FUS/TLS
View all literature mentionsThis monoclonal targets Bassoon mAb (SAP7F407)
View all literature mentionsThis monoclonal targets S-100 (beta-Subunit)
View all literature mentionsThis polyclonal targets VGLUT 1
View all literature mentionsThis polyclonal targets Synaptophysin 1
View all literature mentionsThis polyclonal targets Synaptophysin antibody
View all literature mentionsThis monoclonal targets PSD 95 (PDZ domain)
View all literature mentionsThis monoclonal targets PSD95 antibody [6G6-1C9]
View all literature mentionsThis monoclonal targets Gephyrin
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets GAD65
View all literature mentionsThis monoclonal targets FUS/TLS (4H11)
View all literature mentionsThis polyclonal targets FUS
View all literature mentionsThis polyclonal targets FUS/TLS
View all literature mentionsThis monoclonal targets Bassoon mAb (SAP7F407)
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
