The RefSeq project at the National Center for Biotechnology Information (NCBI) maintains and curates a publicly available database of annotated genomic, transcript, and protein sequence records (http://www.ncbi.nlm.nih.gov/refseq/). The RefSeq project leverages the data submitted to the International Nucleotide Sequence Database Collaboration (INSDC) against a combination of computation, manual curation, and collaboration to produce a standard set of stable, non-redundant reference sequences. The RefSeq project augments these reference sequences with current knowledge including publications, functional features and informative nomenclature. The database currently represents sequences from more than 55,000 organisms (>4800 viruses, >40,000 prokaryotes and >10,000 eukaryotes; RefSeq release 71), ranging from a single record to complete genomes. This paper summarizes the current status of the viral, prokaryotic, and eukaryotic branches of the RefSeq project, reports on improvements to data access and details efforts to further expand the taxonomic representation of the collection. We also highlight diverse functional curation initiatives that support multiple uses of RefSeq data including taxonomic validation, genome annotation, comparative genomics, and clinical testing. We summarize our approach to utilizing available RNA-Seq and other data types in our manual curation process for vertebrate, plant, and other species, and describe a new direction for prokaryotic genomes and protein name management.
Pubmed ID: 26553804 RIS Download
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An integrated application for viewing and analyzing sequence data.
View all literature mentionsA data set of defined genomic sequences used as reference standards for well-characterized genes. These standard nucleotide sequences serve as foundations for locating mutations, establishing conventions for numbering exons and introns, and defining the coordinates of other variations. Sequences are aligned to reference chromosomes. RefSeqGene is a subset of NCBI RefSeq.
View all literature mentionsA database which provides viral and viroid genome sequence data and resource tools. Al viral genomes are downloadable and a complete RefSeq release of viral and viroid genome sequences are available.
View all literature mentionsA repository which supports the search, retrieval, and display of large virus sequence data sets concerning the viruses of Dengue, West Nile, and Influenza. Users can search sequences based on a number of biological and clinical criteria and selected sequences can be downloaded or analyzed using a variety of web-based tools and displays. Virus Variation is an extension of NCBI's Influenza Virus Resource.
View all literature mentionsDatabase for genomes that have been completely sequenced, have active research community to contribute gene-specific information, or that are scheduled for intense sequence analysis. Includes nomenclature, map location, gene products and their attributes, markers, phenotypes, and links to citations, sequences, variation details, maps, expression, homologs, protein domains and external databases. All entries follow NCBI's format for data collections. Content of Entrez Gene represents result of curation and automated integration of data from NCBI's Reference Sequence project (RefSeq), from collaborating model organism databases, and from many other databases available from NCBI. Records are assigned unique, stable and tracked integers as identifiers. Content is updated as new information becomes available.
View all literature mentionsCollection of curated, non-redundant genomic DNA, transcript RNA, and protein sequences produced by NCBI. Provides a reference for genome annotation, gene identification and characterization, mutation and polymorphism analysis, expression studies, and comparative analyses. Accessed through the Nucleotide and Protein databases.
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