The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered report describes the proposed replication plan of key experiments from 'Inhibition of bromodomain and extra terminal (BET) recruitment to chromatin as an effective treatment for mixed-lineage leukemia (MLL)-fusion leukemia' by Dawson and colleagues, published in Nature in 2011 (Dawson et al., 2011). The experiments to be replicated are those reported in Figures 2A, 3D, 4B, 4D and Supplementary Figures 11A-B and 16A. In this study, BET proteins were demonstrated as potential therapeutic targets for modulating aberrant gene expression programs associated with MLL-fusion leukemia. In Figure 2A, the BET bromodomain inhibitor I-BET151 was reported to suppress growth of cells harboring MLL-fusions compared to those with alternate oncogenic drivers. In Figure 3D, treatment of MLL-fusion leukemia cells with I-BET151 resulted in transcriptional suppression of the anti-apoptotic gene BCL2. Figures 4B and 4D tested the therapeutic efficacy of I-BET151 in vivo using mice injected with human MLL-fusion leukemia cells and evaluated disease progression following I-BET151 treatment. The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published in eLife.
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Non-profit organization to increase the openness, integrity, and reproducibility of scientific research. COS priorities are to (1) make the entire scientific workflow accessible, (2) increase scientists' accountability, (3) facilitate good practices by scientists, (4) increase reward for good practices, and (5) promote reproducibility of scientific findings. COS has three primary activities: * Infrastructure development to support the scientific workflow and to connect technologies that support scientific research and communication, such as the Open Science Framework * Community building on open practices among open-source developers, open science researchers, and the broader scientific community, such as the Open Science Collaboration * Metascience, research on scientific practices, such as Reproducibility Project: Psychology and our partnership with Science Exchange, Reproducibility Project: Cancer Biology.
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