Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Publication

The Expression of Tubb2b Undergoes a Developmental Transition in Murine Cortical Neurons.

The Journal of comparative neurology | 2015

The development of the mammalian brain requires the generation, migration, and differentiation of neurons, cellular processes that are dependent on a dynamic microtubule cytoskeleton. Mutations in tubulin genes, which encode for the structural subunits of microtubules, cause detrimental neurological disorders known as the tubulinopathies. The disease spectra associated with different tubulin genes are overlapping but distinct, an observation believed to reflect functional specification of this multigene family. Perturbation of the β-tubulin TUBB2B is known to cause polymicrogyria, pachygyria, microcephaly, and axon guidance defects. Here we provide a detailed analysis of the expression pattern of its murine homolog Tubb2b. The generation and characterization of BAC-transgenic eGFP reporter mouse lines has revealed that it is highly expressed in progenitors and postmitotic neurons during cortical development. This contrasts with the 8-week-old cortex, in which Tubb2b expression is restricted to macroglia, and expression is almost completely absent in mature neurons. This developmental transition in neurons is mirrored in the adult hippocampus and the cerebellum but is not a universal feature of Tubb2b; its expression persists in a population of postmitotic neurons in the 8-week-old retina. We propose that the dynamic spatial and temporal expression of Tubb2b reflects specific functional requirements of the microtubule cytoskeleton.

Pubmed ID: 26105993 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

Agency: Austrian Science Fund FWF, Austria
Id: I 914

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

ANTI-OLIG-2 (antibody)

RRID:AB_570666

This polyclonal targets Oligodendrocute transcription factor 2

View all literature mentions

Anti-PROX1, clone 4G10 (antibody)

RRID:AB_2170714

This monoclonal targets PROX1

View all literature mentions

Ki-67 (antibody)

RRID:AB_442102

This unknown targets Prokaryotic recombinant fusion protein corresponding to a 1086bp Ki67 motif-containing cDNA fragment

View all literature mentions

Anti-phospho-Histone H3 (Ser10) Antibody (antibody)

RRID:AB_310177

This unknown targets phospho-Histone H3 (Ser10)

View all literature mentions

Doublecortin Antibody (C-18) (antibody)

RRID:AB_2088494

This polyclonal targets Doublecortin

View all literature mentions

Anti-Calbindin D-28K (antibody)

RRID:AB_2068336

This polyclonal targets Calbindin D-28K

View all literature mentions

Anti-Tbr2 (antibody)

RRID:AB_10615604

This polyclonal targets Tbr2

View all literature mentions

Sox-2 (Y-17) Antibody (antibody)

RRID:AB_2286684

This polyclonal targets SOX2

View all literature mentions

Glial Fibrillary Acidic Protein (Multipurpose) (antibody)

RRID:AB_10013382

This polyclonal targets GFAP

View all literature mentions

Rabbit Anti-Mouse TBR2 / Eomes Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_778267

This polyclonal targets Mouse TBR2 / Eomes

View all literature mentions

GFP-avictoria (antibody)

RRID:AB_303395

This polyclonal targets GFP

View all literature mentions

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

View all literature mentions

ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions