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To achieve high-level production of non-native isoprenoid products, it requires the metabolic flux to be diverted from the production of sterols to the heterologous metabolic reactions. However, there are limited tools for restricting metabolic flux towards ergosterol synthesis. In the present study, we explored dynamic control of ERG9 expression using different ergosterol-responsive promoters to improve the production of non-native isoprenoids.
Pubmed ID: 25889168 RIS Download
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A software for primer design. The user can choose the target sequence or gene and design primers for the real-time PCR. After, the user can examine the results and choose from the recommended probes.
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