Our hosting provider is investigating network issues. We apologize for the inconvenience.

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133.

Cell | Nov 17, 1989

In this paper, we demonstrate that phosphorylation of CREB at Ser-133 is induced 6-fold in vivo, following treatment of PC12 cells with forskolin. By contrast, no such induction was observed in the kinase A-deficient PC12 line A126-1B2 (A126). Using F9 teratocarcinoma cells, which are unresponsive to cAMP, we initiated a series of transient expression experiments to establish a causal link between phosphorylation of CREB and trans-activation of cAMP-responsive genes. Inactivating the kinase A phosphorylation site by in vitro mutagenesis of the cloned CREB cDNA at Ser-133 completely abolished CREB transcriptional activity. As CREB mutants containing acidic residues in place of the Ser-133 phosphoacceptor were also transcriptionally inactive, these results suggest that phosphorylation of CREB may stimulate transcription by a mechanism other than by simply providing negative charge.

Pubmed ID: 2573431 RIS Download

Mesh terms: Adrenal Gland Neoplasms | Amino Acid Sequence | Animals | Base Sequence | Cell Line | Colforsin | Cyclic AMP | Cyclic AMP Response Element-Binding Protein | DNA-Binding Proteins | Genes | Molecular Sequence Data | Mutation | Peptide Mapping | Pheochromocytoma | Phosphorylation | Rats | Serine | Somatostatin | Transcription, Genetic | Transfection

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.