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Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina.

High-voltage-activated calcium channels are hetero-oligomeric protein complexes that mediate multiple cellular processes, including the influx of extracellular Ca(2+), neurotransmitter release, gene transcription, and synaptic plasticity. These channels consist of a primary α(1) pore-forming subunit, which is associated with an extracellular α(2)δ subunit and an intracellular β auxiliary subunit, which alter the gating properties and trafficking of the calcium channel. The cellular localization of the α(2)δ(3) subunit in the mouse and rat retina is unknown. In this study using RT-PCR, a single band at ∼ 305 bp corresponding to the predicted size of the α(2)δ(3) subunit fragment was found in mouse and rat retina and brain homogenates. Western blotting of rodent retina and brain homogenates showed a single 123-kDa band. Immunohistochemistry with an affinity-purified antibody to the α(2)δ(3) subunit revealed immunoreactive cell bodies in the ganglion cell layer and inner nuclear layer and immunoreactive processes in the inner plexiform layer and the outer plexiform layer. α(2)δ(3) immunoreactivity was localized to multiple cell types, including ganglion, amacrine, and bipolar cells and photoreceptors, but not horizontal cells. The expression of the α(2)δ(3) calcium channel subunit to multiple cell types suggests that this subunit participates widely in Ca-channel-mediated signaling in the retina.

Pubmed ID: 25631988 RIS Download

Mesh terms: Animals | Calcium Channels | Mice | Mice, Inbred C57BL | Nerve Tissue Proteins | Neurons | Rats | Rats, Sprague-Dawley | Retina | Visual Pathways

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Associated grants

  • Agency: BLRD VA, Id: I01 BX000764
  • Agency: NEI NIH HHS, Id: R01 EY004067
  • Agency: NEI NIH HHS, Id: R56 EY004067
  • Agency: NEI NIH HHS, Id: EY04067

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