Searching across hundreds of databases
Amacrine cells comprise ∼ 30 morphological types in the mammalian retina. The synaptic connectivity and function of a few γ-aminobutyric acid (GABA)ergic wide-field amacrine cells have recently been studied; however, with the exception of the rod pathway-specific AII amacrine cell, the connectivity of glycinergic small-field amacrine cells has not been investigated in the mouse retina. Here, we studied the morphology and connectivity pattern of the small-field A8 amacrine cell. A8 cells in mouse retina are bistratified with lobular processes in the ON sublamina and arboreal dendrites in the OFF sublamina of the inner plexiform layer. The distinct bistratified morphology was first visible at postnatal day 8, reaching the adult shape at P13, around eye opening. The connectivity of A8 cells to bipolar cells and ganglion cells was studied by double and triple immunolabeling experiments by using various cell markers combined with synaptic markers. Our data suggest that A8 amacrine cells receive glutamatergic input from both OFF and ON cone bipolar cells. Furthermore, A8 cells are coupled to ON cone bipolar cells by gap junctions, and provide inhibitory input via glycine receptor (GlyR) subunit α1 to OFF cone bipolar cells and to ON A-type ganglion cells. Measurements of spontaneous glycinergic postsynaptic currents and GlyR immunolabeling revealed that A8 cells express GlyRs containing the α2 subunit. The results show that the bistratified A8 cell makes very similar synaptic contacts with cone bipolar cells as the rod pathway-specific AII amacrine cell. However, unlike AII cells, A8 amacrine cells provide glycinergic input to ON A-type ganglion cells.
Pubmed ID: 25630271 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
An Antibody supplier
View all literature mentionsThis polyclonal targets GLRA2
View all literature mentionsThis monoclonal targets CtBP2
View all literature mentionsThis polyclonal targets Human Secretagogin
View all literature mentionsThis unknown targets Mouse Glycine Receptor alpha4
View all literature mentionsThis polyclonal targets VGLUT1
View all literature mentionsThis polyclonal targets GFP (Green Fluorescent Protein)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets GlyR alpha 3
View all literature mentionsThis unknown targets Protein Kinase Calpha
View all literature mentionsThis monoclonal targets syt2b
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsThis polyclonal targets VGLUT1
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsMus musculus with name STOCK Tg(Ier5-EGFP)IG267Gsat/Mmucd from MMRRC.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsThis polyclonal targets Human Secretagogin
View all literature mentionsThis unknown targets Protein Kinase Calpha
View all literature mentionsThis polyclonal targets GlyR alpha 3
View all literature mentionsThis unknown targets Mouse Glycine Receptor alpha4
View all literature mentionsThis polyclonal targets VGLUT1
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsMus musculus with name STOCK Tg(Ier5-EGFP)IG267Gsat/Mmucd from MMRRC.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsThis monoclonal targets syt2b
View all literature mentionsThis polyclonal targets GLRA2
View all literature mentionsThis monoclonal targets CtBP2
View all literature mentionsThis polyclonal targets GFP (Green Fluorescent Protein)
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
