Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.

Nature | Mar 26, 2015

Cell growth and proliferation are tightly linked to nutrient availability. The mechanistic target of rapamycin complex 1 (mTORC1) integrates the presence of growth factors, energy levels, glucose and amino acids to modulate metabolic status and cellular responses. mTORC1 is activated at the surface of lysosomes by the RAG GTPases and the Ragulator complex through a not fully understood mechanism monitoring amino acid availability in the lysosomal lumen and involving the vacuolar H(+)-ATPase. Here we describe the uncharacterized human member 9 of the solute carrier family 38 (SLC38A9) as a lysosomal membrane-resident protein competent in amino acid transport. Extensive functional proteomic analysis established SLC38A9 as an integral part of the Ragulator-RAG GTPases machinery. Gain of SLC38A9 function rendered cells resistant to amino acid withdrawal, whereas loss of SLC38A9 expression impaired amino-acid-induced mTORC1 activation. Thus SLC38A9 is a physical and functional component of the amino acid sensing machinery that controls the activation of mTOR.

Pubmed ID: 25561175 RIS Download

Mesh terms: Amino Acid Transport Systems | Amino Acids | Animals | Cell Line | Humans | Lysosomes | Mice | Monomeric GTP-Binding Proteins | Multiprotein Complexes | Nucleotides | TOR Serine-Threonine Kinases

Research resources used in this publication

None found

Data used in this publication

Associated grants

  • Agency: Austrian Science Fund FWF, Id: P 26682

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

IMEx - The International Molecular Exchange Consortium

Interaction database from an international collaboration between major public interaction data providers who have agreed to share curation effort and: * Develop and work to a single set of curation rules when capturing data from both directly deposited interaction data or from publications in peer-reviewed journals * Capture full details of an interaction in a ����??deep����?? curation model * Perform a complete curation of all protein-protein interactions experimentally demonstrated within a publication * Make these interaction available in a single search interface on a common website * Provide the data in standards compliant download formats * Make all IMEx records freely accessible under the Creative Commons Attribution License Data Availability IMEx partners all produce their own separate resources, which range from all encompassing molecular interaction databases, such as are maintained by IntAct, MINT and DIP, organism-centric resources such as BioGrid or MPIDB or biological domain centric, such as MatrixDB. However additionally to this, they have committed to making records available, via a dedicated PSICQUIC webservice, which have been curated to IMEx rules and are available to the user as a single, non-redundant set of curated publications which can be searched at the IMEx website. Data is made available in standards-compliant tab-deliminated and XML formats, enabling users to visualize the data in a wide range of tools such as Cytoscape and the R Bioconductor software. IMEx partners: * DIP ( (Active) * IntAct ( (Active) * MINT ( (Active) * MPact ( (Active) * MatrixDB ( (Active) * MPIDB ( (Active) * Molecular Connections ( (Active) * BioGRID ( (Observer) * InnateDB ( (Observer) * BIND ( (Inactive) The IMEx consortium is open to the participation of additional partners. The IMEx consortium actively encourages the deposition of data, prior to publication, and will supply unique accession numbers which may be referenced within the final article. Submitters may send their data directly to any of the member databases using a variety of formats, but should conform to the guidelines as to the minimum information required to describe the data.


View all literature mentions


Database of functional genomics experiments including gene expression where you can query and download data collected to MIAME and MINSEQE standards. It includes gene expression data from microarray and high throughput sequencing studies. Gene Expression Atlas contains a subset of curated and re-annotated Archive data which can be queried for individual gene expression under different biological conditions across experiments. The ArrayExpress Archive is one of the three international public repositories of functional genomics data supporting publications. It includes data generated by sequencing or array-based technologies. Data are submitted by users and imported directly from the NCBI Gene Expression Omnibus. The ArrayExpress Archive is closely integrated with the Gene Expression Atlas and the sequence databases at the European Bioinformatics Institute. Advanced queries provided via ontology enabled interfaces include queries based on technology and sample attributes such as disease, cell types and anatomy. The Repository contains data from over 47787 experiments comprising approximately 1361377 assays (March 2014). The majority of the data are array based, but other data types are included, including ultra high-throughput sequencing transcriptomics and epigenetic data. All the data and array designs in ArrayExpress are available for direct download in a number of different formats. Submissions are welcome.


View all literature mentions

CellProfiler Image Analysis Software

CellProfiler cell image analysis software is free and open-source software designed to enable biologists without training in computer vision or programming to quantitatively measure phenotypes from thousands of images automatically. It counts cells and also measures the size, shape, intensity and texture of every cell (and every labeled subcellular compartment) in every image. It was designed for high throughput screening but can perform automated image analysis for images from time-lapse movies and low-throughput experiments. CellProfiler has an increasing number of algorithms to identify and measure properties of neuronal cell types.


View all literature mentions


Software that enables rapid tool creation by providing a robust, pluggable development framework that simplifies and unifies data file access, and performs standard proteomics and LCMS dataset computations.


View all literature mentions


Database that stores curated gene expression DataSets, as well as original Series and Platform records in the Gene Expression Omnibus (GEO) repository. The database can be searched using keywords, organism, DataSet type and authors. DataSet records contain additional resources including cluster tools and differential expression queries.


View all literature mentions