The striatum is the major input nucleus of the basal ganglia involved in reward processing, goal-directed behaviors, habit learning, and motor control. The striatum projects to the basal ganglia output nuclei via the "direct" and "indirect" pathways, which can be distinguished by their projection fields and their opposing effects on behavior. In adult animals, the functional opposition is modulated by the differential actions of D1 and D2 dopamine receptors (D1R, D2R), the expression of which is largely separated between these pathways. To determine whether a similar degree of separation exists earlier in development, we used dual-label immunohistochemistry to map dorsal-striatal D1R and D2R expression at the promoter level in postnatal day 0 (PD0) Drd1a-tdTomato/Drd2-GFP BAC transgenic mice, and at the receptor level by costaining for native D1R and D2R in wildtype (WT) PD0 animals. To assess for potential molecular interactions between D1R and D2R we also employed a recently developed proximity-ligation assay (PLA). Limited coexpression and colocalization of the D1R and D2R proteins was found in clusters of neurons endemic to the "patch" compartment as identified by costaining with tyrosine hydroxylase, but not outside these clusters. Moreover, in contrast to our recent findings where we failed to detect a D1R-D2R PLA signal in the adult striatum, in PD0 striatum we did identify a clear PLA signal for this pair of receptors. This colocalization at close proximity points to a possible role for D1R/D2R-mediated crosstalk in early striatal ontogeny.
Pubmed ID: 25556545 RIS Download
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This monoclonal targets Adenosine Receptor A2a clone 7F6-G5-A2
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View all literature mentionsMus musculus with name B6.Cg-Tg(Drd1a-tdTomato)6Calak/J from IMSR.
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase antibody
View all literature mentionsThis polyclonal targets Choline Acetyltransferase
View all literature mentionsThis monoclonal targets D1 Dopamine Receptor
View all literature mentionsMulti paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionsMus musculus with name STOCK Tg(Drd2-EGFP)S118Gsat/Mmnc from MMRRC.
View all literature mentionsThis polyclonal targets Choline Acetyltransferase
View all literature mentionsThis monoclonal targets Adenosine Receptor A2a clone 7F6-G5-A2
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase antibody
View all literature mentionsThis monoclonal targets D1 Dopamine Receptor
View all literature mentionsThis monoclonal targets Adenosine Receptor A2a clone 7F6-G5-A2
View all literature mentionsThis monoclonal targets D1 Dopamine Receptor
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Adenosine Receptor A2a clone 7F6-G5-A2
View all literature mentionsMus musculus with name B6.Cg-Tg(Drd1a-tdTomato)6Calak/J from IMSR.
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsMus musculus with name STOCK Tg(Drd2-EGFP)S118Gsat/Mmnc from MMRRC.
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase antibody
View all literature mentionsThis polyclonal targets Choline Acetyltransferase
View all literature mentions