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Germ-line transmission of a c-abl mutation produced by targeted gene disruption in ES cells.

A substitution mutation has been introduced into the c-abl locus of murine embryonic stem cells by homologous recombination between exogenously added DNA and the endogenous gene, and these cells have been used to generate chimeric mice. It is shown that the c-abl mutation was transmitted to progeny by several male chimeras. This work demonstrates the feasibility of germ-line transmission of a mutation introduced into a nonselectable autosomal gene by homologous recombination.

Pubmed ID: 2554496


  • Schwartzberg PL
  • Goff SP
  • Robertson EJ


Science (New York, N.Y.)

Publication Data

November 10, 1989

Associated Grants

  • Agency: NCI NIH HHS, Id: P01 CA 23767
  • Agency: NICHD NIH HHS, Id: R01 HD 25208

Mesh Terms

  • Abelson murine leukemia virus
  • Animals
  • Blotting, Southern
  • Cell Line
  • Chimera
  • Cloning, Molecular
  • DNA, Recombinant
  • Female
  • Leukemia Virus, Murine
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Oncogenes
  • Retroviridae Proteins, Oncogenic