Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion of IκB kinase β, the main activating kinase in the inducible NF-κB pathway, selectively in myeloid lineage cells (mIKKβKO) or excitatory neurons (nIKKβKO) to measure synaptic strength at hippocampal Schaffer collaterals during long-term potentiation (LTP) and instrumental conditioning in alert behaving individuals. Resting microglial cells in mIKKβKO mice showed less Iba1-immunoreactivity, and brain IL-1β mRNA levels were selectively reduced compared with controls. Measurement of field excitatory postsynaptic potentials (fEPSPs) evoked by stimulation of the CA3-CA1 synapse in mIKKβKO mice showed higher facilitation in response to paired pulses and enhanced LTP following high frequency stimulation. In contrast, nIKKβKO mice showed normal basic synaptic transmission and LTP induction but impairments in late LTP. To understand the consequences of such impairments in synaptic plasticity for learning and memory, we measured CA1 fEPSPs in behaving mice during instrumental conditioning. IKKβ was not necessary in either microglia or neurons for mice to learn lever-pressing (appetitive behavior) to obtain food (consummatory behavior) but was required in both for modification of their hippocampus-dependent appetitive, not consummatory behavior. Our results show that microglia, through IKKβ and therefore NF-κB activity, regulate hippocampal synaptic plasticity and that both microglia and neurons, through IKKβ, are necessary for animals to modify hippocampus-driven behavior during associative learning.
Pubmed ID: 25297800 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets IKK-beta
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis polyclonal targets IL1B
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis polyclonal targets IkB-a
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsStatistical analysis and scientific graphing software for Windows OS.
View all literature mentionsCustomizable software program for behavioral testing. Users logically order simple text commands to direct experimental work flow and data collection, providing control of chamber components, stimuli, reinforcement mechanisms, data variables, and arrays. Standard pre-written procedures and custom coding solutions are available for purchase.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE.Documented on September 23,2022. A data acquisition and analysis software package for electrophysiology data. Spike2 software offers multi-channel continuous data acquisition and analysis with a multitude of options. This offers flexible usage from a simple chart recorder to complex applications requiring stimulus generation, data capture, scrolling or triggered displays, control of external equipment, and custom analysis. Spike2 software can be used in many fields such as electrophysiology, neurophysiology, cardiovascular and respiratory studies, sports science and pharmacology.
View all literature mentionsMonitor-recognition software
View all literature mentionsLight cycler software for analysis of quatitative real time PCR
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE.Documented on September 23,2022. A data acquisition and analysis software package for electrophysiology data. Spike2 software offers multi-channel continuous data acquisition and analysis with a multitude of options. This offers flexible usage from a simple chart recorder to complex applications requiring stimulus generation, data capture, scrolling or triggered displays, control of external equipment, and custom analysis. Spike2 software can be used in many fields such as electrophysiology, neurophysiology, cardiovascular and respiratory studies, sports science and pharmacology.
View all literature mentionsCustomizable software program for behavioral testing. Users logically order simple text commands to direct experimental work flow and data collection, providing control of chamber components, stimuli, reinforcement mechanisms, data variables, and arrays. Standard pre-written procedures and custom coding solutions are available for purchase.
View all literature mentionsMonitor-recognition software
View all literature mentionsLight cycler software for analysis of quatitative real time PCR
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets IKK-beta
View all literature mentions