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Publication

Quantitative analysis of afferents expressing substance P, calcitonin gene-related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion.

Jin Young Bae | Jae Hyun Kim | Yi Sul Cho | Won Mah | Yong Chul Bae

The Journal of comparative neurology | 2015

Substance P (SP), calcitonin gene-related peptide (CGRP), and isolectin B4 (IB4) are widely used as markers for peripheral neurons with unmyelinated fibers, whereas neurofilament 200 (NF200), and Peripherin are used as markers for neurons with myelinated fibers, and with unmyelinated or small-caliber fibers, respectively. To study the selectivity of these markers for specific neuronal types, we analyzed their expression in neurons in the rat trigeminal ganglion by light- and electron-microscopic immunocytochemistry. Most SP-immunopositive (+), CGRP+, and IB4+ fibers were unmyelinated, but a small fraction (∼5%) were small myelinated fibers (<20 µm(2) in cross-sectional area, equivalent to <5 µm in diameter, Aδ fiber). Similarly, whereas the majority of NF200+ fibers were myelinated, a large fraction (23.9%) were unmyelinated, and whereas the majority of Peripherin+ fibers were unmyelinated and small myelinated, a significant fraction (15.5%) were large myelinated (>20 µm(2) in cross-sectional area, equivalent to >5 µm in diameter, Aβ fiber). Our findings confirm that SP, CGRP, and IB4 can be used as reliable markers for neurons with unmyelinated fibers, and question the suitability of NF200 as a marker for neurons with myelinated fibers, and of Peripherin as a marker for neurons with unmyelinated, or fine-caliber fibers.

Pubmed ID: 25185935 RIS Download

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This is a list of tools and resources that we have found mentioned in this publication.

CGRP (Calcitonin Gene Related Peptide) Antibody (antibody)

RRID:AB_572217

This polyclonal targets rat alpha-CGRP

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Substance P Antibody (antibody)

RRID:AB_572266

This polyclonal targets Substance P

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Anti-Griffonia (Bandeiraea) simplicifolia lectin I (antibody)

RRID:AB_2314660

This unknown targets

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Peripherin (C-19) (antibody)

RRID:AB_2171328

This polyclonal targets Peripherin (C-19)

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Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse (antibody)

RRID:AB_477257

This monoclonal targets Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse

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NTac:SD (organism)

RRID:RGD_1566440

Rattus norvegicus with name NTac:SD from RGD.

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ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

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