Impaired sonic hedgehog (Shh) signaling is involved in the pathology of cortical formation found in neuropsychiatric disorders. However, its role in the specification of human cortical progenitors is not known. Here, we report that Shh is expressed in the human developing cortex at mid-gestation by radial glia cells (RGCs) and cortical neurons. We used RGC cultures, established from the dorsal (cortical) telencephalon of human brain at mid-gestation to study the effect of Shh signaling. Cortical RGCs in vitro maintained their regional characteristics, expressed components of Shh signaling, and differentiated into Nkx2.1, Lhx6, and calretinin-positive (CalR(+)) cells, potential cortical interneuron progenitors. Treatment with exogenous Shh increased the pool of Nkx2.1(+) progenitors, decreased Lhx6 expression, and suppressed the generation of CalR(+) cells. The blockade of endogenous Shh signaling increased the number of CalR(+) cells, but did not affect Nkx2.1 expression, implying the existence of parallel Shh-independent pathways for cortical Nkx2.1 regulation. These results support the idea that, during human brain development, Shh plays an important role in the specification of cortical progenitors. Since direct functional studies in humans are limited, the in vitro system that we established here could be of great interest for modeling the development of human cortical progenitors.
Pubmed ID: 25146370 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Public registry of antibodies with unique identifiers for commercial and non-commercial antibody reagents to give researchers a way to universally identify antibodies used in publications. The registry contains antibody product information organized according to genes, species, reagent types (antibodies, recombinant proteins, ELISA, siRNA, cDNA clones). Data is provided in many formats so that authors of biological papers, text mining tools and funding agencies can quickly and accurately identify the antibody reagents they and their colleagues used. The Antibody Registry allows any user to submit a new antibody or set of antibodies to the registry via a web form, or via a spreadsheet upload.
View all literature mentionsThis polyclonal targets Neuropeptide Y
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets Glyceraldehyde-3-PDH (GAPDH)
View all literature mentionsThis monoclonal targets TTF1
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets GABA
View all literature mentionsThis monoclonal targets beta Actin Loading Control
View all literature mentionsThis unknown targets beta Tubulin
View all literature mentionsThis polyclonal targets Pax-6
View all literature mentionsThis polyclonal targets EMX1
View all literature mentionsThis monoclonal targets Recombinant full length rat MASH1 protein
View all literature mentionsThis monoclonal targets LHX6 antibody produced in mouse
View all literature mentionsThis monoclonal targets Somatostatin
View all literature mentionsThis monoclonal targets Neurofilaments, phospho
View all literature mentionsThis monoclonal targets Parvalbumin
View all literature mentionsThis monoclonal targets Pax6
View all literature mentionsThis monoclonal targets Human Ki67 (MIB-1) (Ki-67; Ki-67 (MIB-13); Ki-67 (MIB-2); Ki-67 (MIB-21); Ki-67 (MIB-24)) Clone Ki67
View all literature mentionsThis monoclonal targets CD15 (Reed-Sternberg Cell Marker)
View all literature mentionsThis monoclonal targets Calretinin
View all literature mentionsThis polyclonal targets BLBP - Neuronal Marker
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis monoclonal targets MAP2
View all literature mentionsThis polyclonal targets DCX, DCLK1
View all literature mentionsThis unknown targets Digoxigenin
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsSoftware for automatic general image analysis. It provides fully automatic analysis of 1-D gels including lane creation, background subtraction, band detection, molecular weight calibration, quantity calibration, and normalization. Editing tools are provided for cropping, rotating, and filtering images.
View all literature mentionsDigital image processing system where microscope settings and processing steps may be adjusted in single user interface. Can acquire images from variety of cameras. Includes software package for capturing, archiving and preparing images for publication. Allows users to visualize and present images in several dimensions. Functionality of imaging toolbox expands constantly with wide range of different modules that are tailored to specific applications or microscope accessories. This resource is duplicated by SCR_018376
View all literature mentionsThis monoclonal targets Glyceraldehyde-3-PDH (GAPDH)
View all literature mentionsThis monoclonal targets TTF1
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Neuropeptide Y
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets TTF1
View all literature mentions