Dendritic arbors are complex neuronal structures that receive and process synaptic inputs. One mechanism regulating dendrite differentiation is Semaphorin/Plexin signaling, specifically through binding of soluble Sema3A to Neuropilin/PlexinA coreceptors. Here we show that the protein Farp1 [FERM, RhoGEF (ARHGEF), and pleckstrin domain protein 1], a Rac1 activator previously identified as a synaptogenic signaling protein, contributes to establishing dendrite tip number and total dendritic branch length in maturing rat neurons and is sufficient to promote dendrite complexity. Aiming to define its upstream partners, our results support that Farp1 interacts with the Neuropilin-1/PlexinA1 complex and colocalizes with PlexinA1 along dendritic shafts. Functionally, Farp1 is required by Sema3A to promote dendritic arborization of hippocampal neurons, and Sema3A regulates dendritic F-actin distribution via Farp1. Unexpectedly, Sema3A also requires neuronal activity to promote dendritic complexity, presumably because silencing neurons leads to a proteasome-dependent reduction of PlexinA1 in dendrites. These results provide new insights into how activity and soluble cues cooperate to refine dendritic morphology through intracellular signaling pathways.
Pubmed ID: 24899721 RIS Download
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Public registry of antibodies with unique identifiers for commercial and non-commercial antibody reagents to give researchers a way to universally identify antibodies used in publications. The registry contains antibody product information organized according to genes, species, reagent types (antibodies, recombinant proteins, ELISA, siRNA, cDNA clones). Data is provided in many formats so that authors of biological papers, text mining tools and funding agencies can quickly and accurately identify the antibody reagents they and their colleagues used. The Antibody Registry allows any user to submit a new antibody or set of antibodies to the registry via a web form, or via a spreadsheet upload.
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