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Contribution of natural antisense transcription to an endogenous siRNA signature in human cells.

BMC genomics | 2014

Eukaryotic cells express a complex layer of noncoding RNAs. An intriguing family of regulatory RNAs includes transcripts from the opposite strand of protein coding genes, so called natural antisense transcripts (NATs). Here, we test the hypothesis that antisense transcription triggers RNA interference and gives rise to endogenous short RNAs (endo-siRNAs).

Pubmed ID: 24410956 RIS Download

Research resources used in this publication

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Antibodies used in this publication

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Associated grants

  • Agency: The Dunhill Medical Trust, United Kingdom
    Id: SA10/0210

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This is a list of tools and resources that we have found mentioned in this publication.


NCBI (tool)

RRID:SCR_006472

A portal to biomedical and genomic information. NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information for the better understanding of molecular processes affecting human health and disease.

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National Antisense Transcript Database (tool)

RRID:SCR_013350

THIS RESOURCE IS NO LONGER IN SERVICE, documented August 19, 2016. A resource that allows users to identify cis-NATs in eleven eukaryotic species, screening eight of these species for the first time and bringing the number of candidate SA pairs in human to 7,246. We construct this free and publicly accessible database that allows researchers to query the dataset.

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Bowtie (tool)

RRID:SCR_005476

Software ultrafast memory efficient tool for aligning sequencing reads. Bowtie is short read aligner.

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HEK293 (tool)

RRID:CVCL_0045

Cell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)

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