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The planarian regeneration transcriptome reveals a shared but temporally shifted regulatory program between opposing head and tail scenarios.

BMC genomics | 2013

Planarians can regenerate entire animals from a small fragment of the body. The regenerating fragment is able to create new tissues and remodel existing tissues to form a complete animal. Thus different fragments with very different starting components eventually converge on the same solution. In this study, we performed an extensive RNA-seq time-course on regenerating head and tail fragments to observe the differences and similarities of the transcriptional landscape between head and tail fragments during regeneration.

Pubmed ID: 24238224 RIS Download

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Associated grants

  • Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
  • Agency: Medical Research Council, United Kingdom

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This is a list of tools and resources that we have found mentioned in this publication.


BLASTX (tool)

RRID:SCR_001653

Web application to search protein databases using a translated nucleotide query. Translated BLAST services are useful when trying to find homologous proteins to a nucleotide coding region. Blastx compares translational products of the nucleotide query sequence to a protein database. Because blastx translates the query sequence in all six reading frames and provides combined significance statistics for hits to different frames, it is particularly useful when the reading frame of the query sequence is unknown or it contains errors that may lead to frame shifts or other coding errors. Thus blastx is often the first analysis performed with a newly determined nucleotide sequence and is used extensively in analyzing EST sequences. This search is more sensitive than nucleotide blast since the comparison is performed at the protein level.

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TBLASTX (tool)

RRID:SCR_011823

A web-based tool used to search translated nucleotide databases using a translated nucleotide query.

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htseq-count (tool)

RRID:SCR_011867

Script distributed with the HT-Seq Python framework for processing RNA-seq or DNA-seq data.

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