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Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington's disease models.

Huntington's disease is caused by an expanded polyglutamine repeat in the huntingtin protein (HTT), but the pathophysiological sequence of events that trigger synaptic failure and neuronal loss are not fully understood. Alterations in N-methyl-D-aspartate (NMDA)-type glutamate receptors (NMDARs) have been implicated. Yet, it remains unclear how the HTT mutation affects NMDAR function, and direct evidence for a causative role is missing. Here we show that mutant HTT redirects an intracellular store of juvenile NMDARs containing GluN3A subunits to the surface of striatal neurons by sequestering and disrupting the subcellular localization of the endocytic adaptor PACSIN1, which is specific for GluN3A. Overexpressing GluN3A in wild-type mouse striatum mimicked the synapse loss observed in Huntington's disease mouse models, whereas genetic deletion of GluN3A prevented synapse degeneration, ameliorated motor and cognitive decline and reduced striatal atrophy and neuronal loss in the YAC128 Huntington's disease mouse model. Furthermore, GluN3A deletion corrected the abnormally enhanced NMDAR currents, which have been linked to cell death in Huntington's disease and other neurodegenerative conditions. Our findings reveal an early pathogenic role of GluN3A dysregulation in Huntington's disease and suggest that therapies targeting GluN3A or pathogenic HTT-PACSIN1 interactions might prevent or delay disease progression.

Pubmed ID: 23852340


  • Marco S
  • Giralt A
  • Petrovic MM
  • Pouladi MA
  • Martínez-Turrillas R
  • Martínez-Hernández J
  • Kaltenbach LS
  • Torres-Peraza J
  • Graham RK
  • Watanabe M
  • Luján R
  • Nakanishi N
  • Lipton SA
  • Lo DC
  • Hayden MR
  • Alberch J
  • Wesseling JF
  • Pérez-Otaño I


Nature medicine

Publication Data

August 7, 2013

Associated Grants

  • Agency: NIEHS NIH HHS, Id: P01 ES016738
  • Agency: NIEHS NIH HHS, Id: P01 ES016738
  • Agency: NICHD NIH HHS, Id: P01 HD029587
  • Agency: NICHD NIH HHS, Id: P01 HD29587
  • Agency: NEI NIH HHS, Id: R01 EY005477
  • Agency: NEI NIH HHS, Id: R01 EY05477

Mesh Terms

  • Animals
  • Behavior, Animal
  • Carrier Proteins
  • Cell Death
  • Disease Models, Animal
  • Gene Deletion
  • HEK293 Cells
  • Humans
  • Huntington Disease
  • Immunoprecipitation
  • Mice
  • Motor Activity
  • Mutant Proteins
  • Neostriatum
  • Nerve Tissue Proteins
  • Neurons
  • Neuropeptides
  • Phosphoproteins
  • Protein Binding
  • Protein Structure, Quaternary
  • Receptors, N-Methyl-D-Aspartate
  • Rotarod Performance Test
  • Synapses